Western Blot (WB)
(TRIM74 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the antibody detected the protein (arrow).)
Rabbit anti-Human TRIM74 Polyclonal Antibody | anti-TRIM74 antibody
TRIM74, CT (TRIM74, TRIM50C, Tripartite motif-containing protein 74, Tripartite motif-containing protein 50C) (Azide free) (HRP)
Gene Names
TRIM74; TRIM50C
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
TRIM74; Polyclonal Antibody; CT (TRIM74; TRIM50C; Tripartite motif-containing protein 74; Tripartite motif-containing protein 50C) (Azide free) (HRP); Tripartite motif-containing protein 50C; anti-TRIM74 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-TRIM74 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
TRIM74 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-193 amino acids from the C-terminal region of human TRIM74.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(TRIM74 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the antibody detected the protein (arrow).)
Western Blot (WB)
(TRIM74 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the antibody detected the protein (arrow).)
Related Product Information for anti-TRIM74 antibody
TRIM50C (Tripartite motif containing 50C) contains one B box-type zinc finger and one RING-type zinc finger. Its function is unknown.
Product Categories/Family for anti-TRIM74 antibody
References
Micale, L., et al. Eur. J. Hum. Genet. 16(9):1038-1049(2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,547 Da
NCBI Official Full Name
tripartite motif-containing protein 74
NCBI Official Synonym Full Names
tripartite motif containing 74
NCBI Official Symbol
TRIM74
NCBI Official Synonym Symbols
TRIM50C
NCBI Protein Information
tripartite motif-containing protein 74; tripartite motif-containing 74; tripartite motif-containing 50C; tripartite motif-containing protein 50C
UniProt Protein Name
Tripartite motif-containing protein 74
Protein Family
UniProt Gene Name
TRIM74
UniProt Synonym Gene Names
TRIM50C
UniProt Entry Name
TRI74_HUMAN
Uniprot Description
TRIM74: Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 7q11.23
Cellular Component: intracellular
Molecular Function: zinc ion binding
Research Articles on TRIM74
Similar Products
Product Notes
The TRIM74 trim74 (Catalog #AAA6358609) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIM74, CT (TRIM74, TRIM50C, Tripartite motif-containing protein 74, Tripartite motif-containing protein 50C) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM74 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TRIM74 trim74 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM74, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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