Rabbit anti-Human WEE1 Polyclonal Antibody | anti-WEE1 antibody

WEE1, Phosphorylated (Ser53) (Wee1-like Protein Kinase, Wee1A Kinase, WEE1hu) (Azide free) (HRP)

Cat. Num. AAA6505964

• Gene Names: WEE1; WEE1A; WEE1hu
• Reactivity: Human
• Applications: ELISA, Western Blot, Dot Blot, Immunohistochemistry
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: WEE1; Polyclonal Antibody; Phosphorylated (Ser53) (Wee1-like Protein Kinase; Wee1A Kinase; WEE1hu) (Azide free) (HRP); anti-WEE1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human WEE1 when phosphorylated at Ser53.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 646

• Applicable Applications for anti-WEE1 antibody: ELISA (EISA), Western Blot (WB), Dot Blot (DB), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:1,000; Dot Blot: 1:500; IHC: 1:50-1:100; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser53 of human WEE1 (KLH).
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-Phospho-Wee1-S53 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma)

Product Categories/Family for anti-WEE1 antibody

Antibodies; Protein Kinases

References

1. Dai, X., et al., J. Invest. Dermatol. 122(6):1356-1364 (2004). 2. Watanabe, N., et al., Proc. Natl. Acad. Sci. USA 101(13):4419-4424 (2004). 3. Yoshida, T., et al., Ann. Oncol. 15(2):252-256 (2004). 4. Kawasaki, H., et al., Oncogene 22(44):6839-6844 (2003). 5. Yuan, H., et al., J. Virol. 77(3):2063-2070 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4507917
• NCBI GeneID: 7465
• NCBI Accession #: NP_003381
• NCBI GenBank Nucleotide #: NP_003381.1
• UniProt Accession #: P30291
• Molecular Weight: 71.597
• NCBI Official Full Name: wee1-like protein kinase isoform 1
• NCBI Official Synonym Full Names: WEE1 G2 checkpoint kinase
• NCBI Official Symbol: WEE1
• NCBI Official Synonym Symbols: WEE1A; WEE1hu
• NCBI Protein Information: wee1-like protein kinase
• UniProt Protein Name: Wee1-like protein kinase
• Protein Family: Wee1-like protein kinase
• UniProt Gene Name: WEE1
• UniProt Synonym Gene Names: WEE1hu
• UniProt Entry Name: WEE1_HUMAN

NCBI Description

This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]

Uniprot Description

Wee1: a protein kinase of the WEE family. A nuclear protein which catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.10.2; Kinase, protein; Protein kinase, Other; Other group; WEE family

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; magnesium ion binding; ATP binding

Biological Process: mitosis; peptidyl-tyrosine phosphorylation; establishment of cell polarity; regulation of cell cycle; cell division; microtubule cytoskeleton organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; blood coagulation; neurite morphogenesis; G1/S transition of mitotic cell cycle