Immunofluorescence (IF)
(Immunofluorescence staining of WBP11 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human WBP11 polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus and cytoplasm.)
Rabbit anti-Human WBP11 Polyclonal Antibody | anti-WBP11 antibody
Anti-WBP11 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
WBP11; Polyclonal Antibody; Anti-WBP11 Antibody; Rabbit Polyclonal; WBP11 Antibody; Rabbit PAb; Antigen Affinity Purified; WW domain binding protein 11; Anti-NPWBP Antibody; Anti-PPP1R165 Antibody; Anti-SIPP1 Antibody; Anti-WBP-11 Antibody; anti-WBP11 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human WBP11
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-WBP11 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ICC/IF: 1:50-1:200
Immunogen
E Coli-derived Human WBP11 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human WBP11 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of WBP11 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human WBP11 polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus and cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of WBP11 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human WBP11 polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus and cytoplasm.)
Related Product Information for anti-WBP11 antibody
WBP11 (WW Domain Binding Protein 11) is a Protein Coding gene. The WBP11 gene, located on 12p12.3, is conserved in chimpanzee, Rhesus monkey, dog, mouse, and rat. This gene encodes a nuclear protein, which colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. This protein has a 95% amino acid sequence identity to the mouse Wbp11 protein. It contains two proline-rich regions that bind to the WW domain of Npw38, a nuclear protein, and thus this protein is also known as Npw38-binding protein NpwBP. WBP11 is widely expressed in testis, bone marrow, and other tissues. Diseases associated with WBP11 include Renpenning Syndrome 1 and Abdominal Obesity-Metabolic Syndrome Quantitative Trait Locus 2.
References
Llorian M, et al. (2005) Nucleocytoplasmic shuttling of the splicing factor sipp1. J Biol Chem 280 (46): 38862-38869.Komuro A, et al. (1999) Association of two nuclear proteins, npw38 and npwbp, via the interaction between the ww domain and a novel proline-rich motif containing glycine and arginine. J Biol Chem 274 (51): 36513-36519.Henning LM, et al. (2017) A new role for fbp21 as regulator of brr2 helicase activity. Nucleic Acids Res 45 (13): 7922-7937.
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Product Notes
The WBP11 (Catalog #AAA8108854) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-WBP11 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WBP11 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:50-1:200. Researchers should empirically determine the suitability of the WBP11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WBP11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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