Rabbit anti-Human WASF3 Polyclonal Antibody | anti-WASF3 antibody

WASF3 Rabbit pAb

Cat. Num. AAA4754932

• Gene Names: WASF3; SCAR3; WAVE3; Brush-1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: WASF3; Polyclonal Antibody; WASF3 Rabbit pAb; SCAR3; WAVE3; Brush-1; anti-WASF3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: -20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-WASF3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:200-1:1000
• Immunogen: Synthetic peptide of human WASF3
• Conjugation: Unconjugated
• Molecular Function: This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. A pseudogene of this gene have been defined on chromosome 6. Alternative splicing results in multiple transcript variants.
• Modification: Unmodified
• Cell Position: Cytoplasm, Cytoskeleton
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-3: A172, 293T and Hepg2 cell lysates, Primary antibody: 162489(WASF3 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 162489(WASF3 Antibody) at dilution 1/25. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 162489(WASF3 Antibody) at dilution 1/25. (Original magnification: ×200))

Product Categories/Family for anti-WASF3 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 13699803
• NCBI GeneID: 10810
• NCBI Accession #: NP_006637
• NCBI GenBank Nucleotide #: NM_006646
• UniProt Accession #: Q9UPY6
• Molecular Weight: 55kDa
• NCBI Official Full Name: wiskott-Aldrich syndrome protein family member 3 isoform 1
• NCBI Official Synonym Full Names: WASP family member 3
• NCBI Official Symbol: WASF3
• NCBI Official Synonym Symbols: SCAR3; WAVE3; Brush-1
• NCBI Protein Information: wiskott-Aldrich syndrome protein family member 3
• UniProt Protein Name: Wiskott-Aldrich syndrome protein family member 3
• Protein Family: Wiskott-Aldrich syndrome protein family
• UniProt Gene Name: WASF3
• UniProt Synonym Gene Names: KIAA0900; SCAR3; WAVE3; WASP family protein member 3
• UniProt Entry Name: WASF3_HUMAN

NCBI Description

This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. A pseudogene of this gene have been defined on chromosome 6. Alternative splicing results in multiple transcript variants [provided by RefSeq, May 2014]

Uniprot Description

WASF3: Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. Binds actin and the Arp2/3 complex. Expressed in ovary and brain. Belongs to the SCAR/WAVE family.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: cytoskeleton; lamellipodium; cytoplasm

Molecular Function: actin binding

Biological Process: positive regulation of myelination; lamellipodium biogenesis; regulation of cell shape; actin filament polymerization; protein complex assembly; cytoskeleton organization and biogenesis; oligodendrocyte development