Rabbit anti-Human VTCN1 Polyclonal Antibody | anti-VTCN1 antibody

VTCN1, ID (VTCN1, B7H4, V-set domain-containing T-cell activation inhibitor 1, B7h.5, Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x) (FITC)

Cat. Num. AAA6362909

• Gene Names: VTCN1; B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291; RP11-229A19.4
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: VTCN1; Polyclonal Antibody; ID (VTCN1; B7H4; V-set domain-containing T-cell activation inhibitor 1; B7h.5; Immune costimulatory protein B7-H4; Protein B7S1; T-cell costimulatory molecule B7x) (FITC); T-cell costimulatory molecule B7x; anti-VTCN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-VTCN1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: VTCN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79~108 amino acids from the Central region of human VTCN1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of VTCN1 Antibody (Center) in T47D cell line lysates (35ug/lane). VTCN1 (arrow) was detected using the purified Pab.)

Related Product Information for anti-VTCN1 antibody

VTCN1 belongs to the B7 family (see CD80; MIM 112203) of costimulatory proteins. These proteins are expressed on the surface of antigen-presenting cells and interact with ligands (e.g., CD28; MIM 186760) on T lymphocytes.

Product Categories/Family for anti-VTCN1 antibody

Antibodies; Proteins

References

Xue, Q., et al. Stem Cells Dev. 19(1):27-38(2010). Cheng, L., et al. Int. J. Gynecol. Cancer 19(9):1481-1486(2009). Hinks, A., et al. Arthritis Rheum. 60(1):258-263(2009). Zhang, J., et al. BMC Cancer 9, 394 (2009): Thompson, R.H., et al. Cancer Res. 68(15):6054-6058(2008). Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003). Zang, X., et al. Proc. Natl. Acad. Sci. U.S.A. 100(18):10388-10392(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 70997756
• NCBI GeneID: 79679
• UniProt Accession #: Q7Z7D3; Q0GN76; Q45VN0; Q5WPZ3; Q6P097; Q9H6B2
• Molecular Weight: 30,878 Da
• NCBI Official Full Name: B7-H4
• NCBI Official Synonym Full Names: V-set domain containing T cell activation inhibitor 1
• NCBI Official Symbol: VTCN1
• NCBI Official Synonym Symbols: B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291; RP11-229A19.4
• NCBI Protein Information: V-set domain-containing T-cell activation inhibitor 1; B7 family member, H4; B7 superfamily member 1; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; immune costimulatory protein B7-H4
• UniProt Protein Name: V-set domain-containing T-cell activation inhibitor 1
• Protein Family: V-set domain containing T-cell activation inhibitor
• UniProt Gene Name: VTCN1
• UniProt Synonym Gene Names: B7H4
• UniProt Entry Name: VTCN1_HUMAN

NCBI Description

This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

VTCN1: Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor- associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: receptor binding

Biological Process: response to protozoan; immune system process; positive regulation of T cell proliferation; negative regulation of T cell activation