Rabbit VPS41 Polyclonal Antibody | anti-VPS41 antibody

VPS41 Antibody

Cat. Num. AAA9611546

• Gene Names: VPS41; HVPS41; HVSP41; hVps41p
• Reactivity: Human, Monkey; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: VPS41; Polyclonal Antibody; VPS41 Antibody; HVPS41; hVps41p; HVSP41; S53; Vacuolar assembly protein 41; Vacuolar protein sorting 41 (yeast homolog); Vacuolar protein sorting 41 (yeast); Vacuolar protein sorting 41 homolog (S. cerevisiae); Vacuolar protein sorting associated protein 41 homolog; vacuolar protein sorting-associated protein 41 homolog; VPS 41; anti-VPS41 antibody

• Host: Rabbit
• Reactivity: Human, Monkey; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: VPS41 Antibody detects endogenous levels of VPS41.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 854

• Applicable Applications for anti-VPS41 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human VPS41.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Endosome; Golgi Apparatus; Lysosome
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from COS-7, using VPS41 Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, using VPS41 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-VPS41 antibody

Description: Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act in part as a core component of the putative HOPS endosomal tethering complex is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes (PubMed:23351085). Involved in homotypic vesicle fusions between late endosomes and in heterotypic fusions between late endosomes and lysosomes implicated in degradation of endocytosed cargo (PubMed:9159129, PubMed:23167963, PubMed:25445562, PubMed:25908847). Required for fusion of autophagosomes with lysosomes (PubMed:25783203). May link the HOPS complex to endosomal Rab7 via its association with RILP and to lysosomal membranes via its association with ARL8B, suggesting that these interactions may bring the compartments to close proximity for fusion (PubMed:25445562, PubMed:25908847). Involved in the direct trans-Golgi network to late endosomes transport of lysosomal membrane proteins independently of HOPS (PubMed:23322049). Involved in sorting to the regulated secretory pathway presumably implicating the AP-3 adaptor complex (By similarity). May play a role in HOPS-independent function in the regulated secretory pathway (PubMed:24210660).

Subunit Structure: Component of the putative homotypic fusion and vacuole protein sorting (HOPS) complex; the core of which composed of the class C Vps proteins VPS11, VPS16, VPS18 and VPS33A, is associated with VPS39 and VPS41 (PubMed:23351085, PubMed:25445562, PubMed:25908847). Interacts with RILP, MON1B (PubMed:20434987, PubMed:25445562). Interacts with ARL8B (GTP-bound form); involved in recruitment to lysosomes and probably hierarchial assembly of the HOPS complex at lysosomal membranes (PubMed:25908847). In vitro can self-assemble into a lattice (PubMed:24210660). Associates with adaptor protein complex 3 (AP-3) and clathrin:AP-3 complexes (PubMed:21411634).

Similarity: Belongs to the VPS41 family.

NCBI and Uniprot Product Information

• NCBI GI #: 114199475
• NCBI GeneID: 27072
• NCBI Accession #: NP_055211.2
• NCBI GenBank Nucleotide #: NP_055211.2
• UniProt Accession #: P49754
• Molecular Weight: Observed: 94 kDa; Predicted: 99 kDa
• NCBI Official Full Name: vacuolar protein sorting-associated protein 41 homolog isoform 1
• NCBI Official Synonym Full Names: VPS41 subunit of HOPS complex
• NCBI Official Symbol: VPS41
• NCBI Official Synonym Symbols: HVPS41; HVSP41; hVps41p
• NCBI Protein Information: vacuolar protein sorting-associated protein 41 homolog
• UniProt Protein Name: Vacuolar protein sorting-associated protein 41 homolog
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS41
• UniProt Entry Name: VPS41_HUMAN

NCBI Description

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human ortholog of yeast Vps41 protein which is also conserved in Drosophila, tomato, and Arabidopsis. Expression studies in yeast and human indicate that this protein may be involved in the formation and fusion of transport vesicles from the Golgi. Several transcript variants encoding different isoforms have been described for this gene, however, the full-length nature of not all is known. [provided by RefSeq, Jul 2008]

Uniprot Description

VPS41: Required for vacuolar assembly and vacuolar traffic. Belongs to the VPS41 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Vesicle

Chromosomal Location of Human Ortholog: 7p14-p13

Cellular Component: microtubule cytoskeleton; membrane; lysosomal membrane; late endosome; early endosome; Golgi-associated vesicle; cytosol

Molecular Function: protein binding; GTPase binding; zinc ion binding; microtubule binding

Biological Process: vesicle-mediated transport; protein targeting to vacuole; vacuole fusion, non-autophagic; Golgi vesicle transport