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IHC (Immunohistochemistry)
(UBE2I/UBC9 Antibody-Immunohistochemistry of paraffin-embedded human breast cancer using UBE2I antibody at dilution of 1:200 (400x lens).)
Rabbit UBE2I/UBC9 Polyclonal Antibody | anti-UBE2I antibody
UBE2I/UBC9 Rabbit anti-Human Polyclonal Antibody
Gene Names
UBE2I; P18; UBC9; C358B7.1
Reactivity
Mouse, Rat, Human
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Affinity purified
Synonyms
UBE2I/UBC9; Polyclonal Antibody; UBE2I/UBC9 Rabbit anti-Human Polyclonal Antibody; IHC-plus UBE2I/UBC9 Antibody; UBE2I; C358B7.1; SUMO-conjugating enzyme UBC9; UBC9; SUMO-protein ligase; UBCE9; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-protein ligase I; p18; SUMO-1-protein ligase; Ubiquitin conjugating enzyme 9; Ubiquitin-protein ligase E2I; anti-UBE2I antibody
Host
Rabbit
Reactivity
Mouse, Rat, Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human UBE2I/UBC9
Purity/Purification
Affinity purified
Form/Format
PBS, pH7.3, 0.02% Sodium Azide, 50% Glycerol
Applicable Applications for anti-UBE2I antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)
Application Notes
IHC-P: 1:200
WB: 1:500-1:2000
The predicted MW is 18kDa, while the observed MW by Western blot was 18kDa.
WB: 1:500-1:2000
The predicted MW is 18kDa, while the observed MW by Western blot was 18kDa.
Target
Human UBE2I/UBC9
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-158 of human UBE2I (NP_003336.1). MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze-thaw cycles.
IHC (Immunohistochemistry)
(UBE2I/UBC9 Antibody-Immunohistochemistry of paraffin-embedded human breast cancer using UBE2I antibody at dilution of 1:200 (400x lens).)
IHC (Immunohistochemistry)
(UBE2I/UBC9 Antibody-Immunohistochemistry of paraffin-embedded human breast cancer using UBE2I antibody at dilution of 1:200 (400x lens).)
IHC (Immunohistchemistry)
(UBE2I/UBC9 Antibody-Immunohistochemistry of paraffin-embedded human esophageal cancer using UBE2I antibody at dilution of 1:200 (400x lens).)
IHC (Immunohistchemistry)
(UBE2I/UBC9 Antibody-Immunohistochemistry of paraffin-embedded human esophageal cancer using UBE2I antibody at dilution of 1:200 (400x lens).)
IHC (Immunohistochemistry)
(UBE2I/UBC9 Antibody-Immunohistochemistry of paraffin-embedded rat lung tissue.)
IHC (Immunohistochemistry)
(UBE2I/UBC9 Antibody-Immunohistochemistry of paraffin-embedded rat lung tissue.)
IHC (Immunohistochemistry)
(UBE2I/UBC9 Antibody-Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(UBE2I/UBC9 Antibody-Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(UBE2I/UBC9 Antibody-Western blot analysis of extracts of various cell lines, using UBE2I antibody.)
WB (Western Blot)
(UBE2I/UBC9 Antibody-Western blot analysis of extracts of various cell lines, using UBE2I antibody.)
WB (Western Blot)
(UBE2I/UBC9 Antibody-Western blot analysis of extracts of various cell lines.)
WB (Western Blot)
(UBE2I/UBC9 Antibody-Western blot analysis of extracts of various cell lines.)
ICC (Immunocytochemistry)
(UBE2I/UBC9 Antibody-Immunofluorescence analysis of U2OS cell using UBE2I antibody. Blue: DAPI for nuclear staining.)
ICC (Immunocytochemistry)
(UBE2I/UBC9 Antibody-Immunofluorescence analysis of U2OS cell using UBE2I antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-UBE2I antibody
UBC9 antibody is an unconjugated rabbit polyclonal antibody to UBC9 (UBE2I) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
158
NCBI Official Full Name
SUMO-conjugating enzyme UBC9
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2I
NCBI Official Symbol
UBE2I
NCBI Official Synonym Symbols
P18; UBC9; C358B7.1
NCBI Protein Information
SUMO-conjugating enzyme UBC9; SUMO-protein ligase; SUMO-1-protein ligase; ubiquitin-protein ligase I; ubiquitin carrier protein 9; ubiquitin carrier protein I; ubiquitin-protein ligase E2I; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme UbcE
UniProt Protein Name
SUMO-conjugating enzyme UBC9
UniProt Gene Name
UBE2I
UniProt Synonym Gene Names
UBC9; UBCE9
UniProt Entry Name
UBC9_HUMAN
Similar Products
Product Notes
The UBE2I ube2i (Catalog #AAA21313) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBE2I/UBC9 Rabbit anti-Human Polyclonal Antibody reacts with Mouse, Rat, Human and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2I/UBC9 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB). IHC-P: 1:200 WB: 1:500-1:2000 The predicted MW is 18kDa, while the observed MW by Western blot was 18kDa. Researchers should empirically determine the suitability of the UBE2I ube2i for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2I/UBC9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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