Rabbit VPS39 Polyclonal Antibody | anti-VPS39 antibody

Rabbit Polyclonal to Human VPS39

Cat. Num. AAA243951

• Gene Names: VPS39; TLP; VAM6; hVam6p
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: VPS39; Polyclonal Antibody; Rabbit Polyclonal to Human VPS39; Anti-VPS39 Antibody (C-Terminus) IHC-plus; HVam6p; KIAA0770; TRAP1-like protein; Vam6/Vps39-like protein; VAM6; TLP; Human VPS39; anti-VPS39 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Human VPS39. At least four isoforms of VPS39 are known to exist; this antibody will detect all four. VPS39 antibody is predicted not to cross-react with TRAP1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 886

• Applicable Applications for anti-VPS39 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (5 ug/ml), WB (0.5 - 1 ug/ml); Usage: Tested in Western blot using rat livery lysates and in IHC-P with FFPE human samples.
• Target Species: Human
• Immunogen Description: A 18 amino acid peptide near the carboxy terminus of human VPS39
• Immunogen Type: Synthetic peptide
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-VPS39 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of VPS39 in mouse liver tissue with VPS39 antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot analysis of VPS39 in rat liver tissue lysate with VPS39 antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 667478007
• NCBI GeneID: 23339
• NCBI Accession #: NP_001288067.1
• NCBI GenBank Nucleotide #: NM_001301138.1
• UniProt Accession #: Q96JC1; O94869; Q71SQ6; Q7Z3V3; Q96B93; Q96RM0
• Molecular Weight: 100,769 Da
• NCBI Official Full Name: vam6/Vps39-like protein isoform a
• NCBI Official Synonym Full Names: vacuolar protein sorting 39 homolog (S. cerevisiae)
• NCBI Official Symbol: VPS39
• NCBI Official Synonym Symbols: TLP; VAM6; hVam6p
• NCBI Protein Information: vam6/Vps39-like protein; TRAP1-like protein
• UniProt Protein Name: Vam6/Vps39-like protein
• Protein Family: Vam6/Vps39-like protein
• UniProt Gene Name: VPS39
• UniProt Synonym Gene Names: KIAA0770; TLP; VAM6; hVam6p
• UniProt Entry Name: VPS39_HUMAN

NCBI Description

This gene encodes a protein that may promote clustering and fusion of late endosomes and lysosomes. The protein may also act as an adaptor protein that modulates the transforming growth factor-beta response by coupling the transforming growth factor-beta receptor complex to the Smad pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

VPS39: May play a role in clustering and fusion of late endosomes and lysosomes. Regulator of TGF-beta/activin signaling, inhibiting SMAD3- and activating SMAD2-dependent transcription. Acts by interfering with SMAD3/SMAD4 complex formation, this would lead to inhibition of SMAD3-dependent transcription and relieve SMAD3 inhibition of SMAD2-dependent promoters, thus increasing SMAD2-dependent transcription. Does not affect TGF-beta-induced SMAD2 or SMAD3 phosphorylation, nor SMAD2/SMAD4 complex formation. Belongs to the VAM6/VPS39 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein regulator, misc.; Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 15q15.1

Cellular Component: lysosomal membrane; late endosome membrane

Biological Process: vesicle-mediated transport; intracellular protein transport; regulation of catalytic activity