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Western Blot (WB) (Gel: 12%SDS-PAGE Lysate: 40 μ g Lane: Human fetal liver tissue Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds)

Rabbit anti-Human VPS25 Polyclonal Antibody | anti-VPS25 antibody

VPS25 Antibody

Gene Names
VPS25; DERP9; EAP20; FAP20
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
VPS25; Polyclonal Antibody; VPS25 Antibody; DERP9; EAP20; FAP20; anti-VPS25 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total VPS25 protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1.3 mg/ml (varies by lot)
Sequence Length
176
Applicable Applications for anti-VPS25 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:200-1:1000
Immunohistochemistry: 1:25-1:100
Immunogen
Full length fusion protein of human VPS25
Immunogen Type
Protein
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane: Human fetal liver tissue Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds)

Western Blot (WB) (Gel: 12%SDS-PAGE Lysate: 40 μ g Lane: Human fetal liver tissue Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds)
Related Product Information for anti-VPS25 antibody
This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1.
Product Categories/Family for anti-VPS25 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,748 Da
NCBI Official Full Name
vacuolar protein-sorting-associated protein 25
NCBI Official Synonym Full Names
vacuolar protein sorting 25 homolog
NCBI Official Symbol
VPS25
NCBI Official Synonym Symbols
DERP9; EAP20; FAP20
NCBI Protein Information
vacuolar protein-sorting-associated protein 25
UniProt Protein Name
Vacuolar protein-sorting-associated protein 25
UniProt Gene Name
VPS25
UniProt Synonym Gene Names
DERP9; EAP20; hVps25
UniProt Entry Name
VPS25_HUMAN

Uniprot Description

VPS25: Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses. Belongs to the VPS25 family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: cytoplasm; cytosol; endosome membrane; nucleolus; nucleus

Molecular Function: protein binding; protein homodimerization activity; protein N-terminus binding; structural molecule activity

Biological Process: autophagy; endosome transport; protein targeting to vacuole during ubiquitin-dependent protein catabolic process via the MVB pathway

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Product Notes

The VPS25 vps25 (Catalog #AAA9416429) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VPS25 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VPS25 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:200-1:1000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the VPS25 vps25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VPS25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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