Rabbit anti-Mouse VPS25 Polyclonal Antibody | anti-VPS25 antibody

VPS25, CT (VPS25, DERP9, EAP20, Vacuolar protein-sorting-associated protein 25, Dermal papilla-derived protein 9, ELL-associated protein of 20kD, ESCRT-II complex subunit VPS25) (Azide free) (HRP)

Cat. Num. AAA6362723

• Gene Names: VPS25; DERP9; EAP20; FAP20
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: VPS25; Polyclonal Antibody; CT (VPS25; DERP9; EAP20; Vacuolar protein-sorting-associated protein 25; Dermal papilla-derived protein 9; ELL-associated protein of 20kD; ESCRT-II complex subunit VPS25) (Azide free) (HRP); ESCRT-II complex subunit VPS25; anti-VPS25 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-VPS25 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: VPS25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-144 amino acids from the C-terminal region of human VPS25.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(VPS25 Antibody (C-term) western blot analysis in kidney cell line lysates (35ug/lane).This demonstrates the VPS25 antibody detected the VPS25 protein (arrow).)

Related Product Information for anti-VPS25 antibody

VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).

Product Categories/Family for anti-VPS25 antibody

Antibodies; Proteins

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Im, Y.J., et al. Dev. Cell 17(2):234-243(2009). Lamesch, P., et al. Genomics 89(3):307-315(2007). Bowers, K., et al. J. Biol. Chem. 281(8):5094-5105(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 14150155
• NCBI GeneID: 84313
• NCBI Accession #: NP_115729.1
• NCBI GenBank Nucleotide #: NM_032353.3
• UniProt Accession #: Q9BRG1; B2R581
• Molecular Weight: 20,748 Da
• NCBI Official Full Name: vacuolar protein-sorting-associated protein 25
• NCBI Official Synonym Full Names: vacuolar protein sorting 25 homolog (S. cerevisiae)
• NCBI Official Symbol: VPS25
• NCBI Official Synonym Symbols: DERP9; EAP20; FAP20
• NCBI Protein Information: vacuolar protein-sorting-associated protein 25; ELL-associated protein of 20 kDa; ESCRT-II complex subunit VPS25; dermal papilla-derived protein 9
• UniProt Protein Name: Vacuolar protein-sorting-associated protein 25
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS25
• UniProt Synonym Gene Names: DERP9; EAP20; hVps25
• UniProt Entry Name: VPS25_HUMAN

NCBI Description

This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1. [provided by RefSeq, Jul 2013]

Uniprot Description

VPS25: Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses. Belongs to the VPS25 family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: nucleoplasm; mitochondrion; nucleolus; endosome membrane; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; structural molecule activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; protein targeting to vacuole during ubiquitin-dependent protein catabolic process via the MVB pathway; endosome transport