Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Kidney-. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Goat VPS11 Polyclonal Antibody | anti-VPS11 antibody
Goat anti-VPS11 Antibody
Immunohistochemistry: In paraffin embedded Human Kidney shows brushborder staning in PCT cells and Human Pancreas shows cytoplasm staining of beta cells in islets of Langerhans. Recommended concentration, 3-6ug/ml.
Western Blot: Preliminary experiments gave an approx 75kDa band in human duodenum, colon and placenta lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 108kDa according to NP_068375.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide.
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Kidney-. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Kidney-. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.)
Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.)
NCBI and Uniprot Product Information
NCBI Description
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Uniprot Description
VPS11: May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Belongs to the VPS11 family.
Protein type: Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 11q23
Cellular Component: autophagic vacuole; clathrin-coated vesicle; early endosome; endocytic vesicle; endosome; late endosome; late endosome membrane; lysosomal membrane; lysosome
Molecular Function: nucleotide binding; protein binding; protein binding, bridging; protein domain specific binding; syntaxin binding; zinc ion binding
Biological Process: autophagy; endosomal vesicle fusion; endosome organization and biogenesis; endosome to lysosome transport; intracellular protein transport; lysosome organization and biogenesis; regulation of protein stability; vesicle docking during exocytosis
Research Articles on VPS11
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Product Notes
The VPS11 vps11 (Catalog #AAA421433) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-VPS11 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's VPS11 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Kidney shows brushborder staning in PCT cells and Human Pancreas shows cytoplasm staining of beta cells in islets of Langerhans. Recommended concentration, 3-6ug/ml. Western Blot: Preliminary experiments gave an approx 75kDa band in human duodenum, colon and placenta lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 108kDa according to NP_068375.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the VPS11 vps11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HALQEKDTQT KLE. It is sometimes possible for the material contained within the vial of "VPS11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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