Rabbit anti-Human VPS11 Polyclonal Antibody | anti-VPS11 antibody

VPS11, CT (VPS11, RNF108, Vacuolar protein sorting-associated protein 11 homolog, RING finger protein 108) (Biotin)

Cat. Num. AAA6362710

• Gene Names: VPS11; END1; PEP5; RNF108; hVPS11
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: VPS11; Polyclonal Antibody; CT (VPS11; RNF108; Vacuolar protein sorting-associated protein 11 homolog; RING finger protein 108) (Biotin); RING finger protein 108; anti-VPS11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-VPS11 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: VPS11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 771-800 amino acids from the C-terminal region of human VPS11.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(VPS11 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the VPS11 antibody detected the VPS11 protein (arrow).)

Related Product Information for anti-VPS11 antibody

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.

Product Categories/Family for anti-VPS11 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Bailey, S.D., et al. Diabetes Care (2010). In press: Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Zhu, G.D., et al. Mol. Biol. Cell 20(4):1223-1240(2009). Wan, D., et al. Proc. Natl. Acad. Sci. U.S.A. 101(44):15724-15729(2004). Lehner, B., et al. Genome Res. 14(7):1315-1323(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 17978477
• NCBI GeneID: 55823
• NCBI Accession #: NP_068375.3
• NCBI GenBank Nucleotide #: NM_021729.5
• UniProt Accession #: Q9H270; Q8WY89; Q96EP8; Q9H6D9; Q9HCS6
• Molecular Weight: 107,837 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 11 homolog isoform 1
• NCBI Official Synonym Full Names: vacuolar protein sorting 11 homolog (S. cerevisiae)
• NCBI Official Symbol: VPS11
• NCBI Official Synonym Symbols: END1; PEP5; RNF108; hVPS11
• NCBI Protein Information: vacuolar protein sorting-associated protein 11 homolog; RING finger protein 108
• UniProt Protein Name: Vacuolar protein sorting-associated protein 11 homolog
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS11
• UniProt Synonym Gene Names: RNF108; hVPS11
• UniProt Entry Name: VPS11_HUMAN

NCBI Description

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

VPS11: May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Belongs to the VPS11 family.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: late endosome membrane; lysosome; endocytic vesicle; lysosomal membrane; late endosome; endosome

Molecular Function: protein binding; syntaxin binding; zinc ion binding; nucleotide binding

Biological Process: vesicle-mediated transport; intracellular protein transport