Goat Vitamin D-binding protein/GC Polyclonal Antibody | anti-GC antibody

Goat anti-Vitamin D-binding protein/GC Antibody

Cat. Num. AAA420897

• Gene Names: GC; DBP; GRD3; VDBG; VDBP; GcMAF; DBP/GC; Gc-MAF; HEL-S-51
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: Vitamin D-binding protein/GC; Polyclonal Antibody; Goat anti-Vitamin D-binding protein/GC Antibody; Goat Anti-VCBP / GC (aa 18 - 30) Antibody; GC; vitamin D-binding protein; group-specific component (vitamin D binding protein); DBP; DBP/GC; VDBG; VDBP; vitamin D binding protein; vitamin D-binding alpha-globulin; vitamin D-binding protein/group specific component; GC antibody; vitamin D-binding protein antibody; group-specific component (vitamin D binding protein) antibody; DBP antibody; DBP/GC antibody; VDBG antibody; VDBP antibody; vitamin D binding protein antibody; vitamin D-binding alpha-globulin antibody; vitamin D-binding protein/group specific component antibody; Vitamin D-binding protein; Vitamin D-binding protein / GC; anti-GC antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: CDNLSTKNSKFED
• Sequence Length: 474

• Applicable Applications for anti-GC antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 2000.; Western Blot: Approx 56kDa band observed in Human Lung lysates (calculated MW of 52.9kDa according to NP_000574.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (DiMartino and Kew, J Immunol.1999 Aug 15;163(4): 2135-42; PMID: 10438954). Recommended concentration: 0.1-0.3ug/ml.
• Immunogen: Peptide with sequence CDNLSTKNSKFED, from the internal region of the protein sequence according to NP_000574.2.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0. 03ug/ml) staining of Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 32483410
• NCBI GeneID: 2638
• NCBI Accession #: NP_000574.2
• NCBI GenBank Nucleotide #: NM_000583.3
• Molecular Weight: 55,123 Da
• NCBI Official Full Name: vitamin D-binding protein isoform 1
• NCBI Official Synonym Full Names: GC, vitamin D binding protein
• NCBI Official Symbol: GC
• NCBI Official Synonym Symbols: DBP; GRD3; VDBG; VDBP; GcMAF; DBP/GC; Gc-MAF; HEL-S-51
• NCBI Protein Information: vitamin D-binding protein
• UniProt Protein Name: Vitamin D-binding protein
• Protein Family: Vitamin D-binding protein
• UniProt Gene Name: GC
• UniProt Synonym Gene Names: DBP-maf
• UniProt Entry Name: VTDB_HUMAN

NCBI Description

The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

GC: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q12-q13

Cellular Component: axon; cytosol; extracellular region; extracellular space; lysosomal lumen; perinuclear region of cytoplasm

Molecular Function: actin binding; vitamin D binding; vitamin transporter activity

Biological Process: female pregnancy; lactation; response to estradiol stimulus; response to nutrient levels; vitamin D metabolic process; vitamin transport

Disease: Graves Disease, Susceptibility To, 1