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Western Blot (WB) (VILL Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the VILL antibody detected the VILL protein (arrow).)

Rabbit anti-Human VILL Polyclonal Antibody | anti-VILL antibody

VILL Antibody (Center)

Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
VILL; Polyclonal Antibody; VILL Antibody (Center); Villin-like protein; anti-VILL antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This VILL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 385-412 amino acids from the Central region of human VILL.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
385-412
Sequence Length
856
Applicable Applications for anti-VILL antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(VILL Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the VILL antibody detected the VILL protein (arrow).)

Western Blot (WB) (VILL Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the VILL antibody detected the VILL protein (arrow).)

Immunohistochemistry (IHC)

(VILL Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VILL Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (VILL Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VILL Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of VILL Antibody (Center) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF) (Confocal immunofluorescent analysis of VILL Antibody (Center) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(VILL Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (VILL Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-VILL antibody
The protein encoded by this gene belongs to the
villin/gelsolin family. It contains 6 gelsolin-like repeats and a
headpiece domain. It may play a role in actin-bundling. [provided
by RefSeq].
Product Categories/Family for anti-VILL antibody
References
Piana, S., et al. J. Mol. Biol. 375(2):460-470(2008)
Yamamichi, N., et al. Cancer Res. 67(22):10727-10735(2007)
Tomar, A., et al. J. Biol. Chem. 281(42):31972-31986(2006)
Ishikawa, S., et al. DNA Res. 4(1):35-43(1997)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
95907
NCBI Official Full Name
villin-like protein
NCBI Official Synonym Full Names
villin-like
NCBI Official Symbol
VILL
NCBI Protein Information
villin-like protein
UniProt Protein Name
Villin-like protein
Protein Family
UniProt Gene Name
VILL
UniProt Entry Name
VILL_HUMAN

NCBI Description

The protein encoded by this gene belongs to the villin/gelsolin family. It contains 6 gelsolin-like repeats and a headpiece domain. It may play a role in actin-bundling. [provided by RefSeq, Jul 2008]

Uniprot Description

VILL: Possible tumor suppressor. Belongs to the villin/gelsolin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: actin cytoskeleton

Molecular Function: structural constituent of cytoskeleton; actin binding

Biological Process: actin filament capping; cytoskeleton organization and biogenesis

Research Articles on VILL

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Product Notes

The VILL vill (Catalog #AAA9210951) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 385-412. The VILL Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VILL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the VILL vill for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VILL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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