Rabbit anti-Human, Mouse VILIP3 Polyclonal Antibody | anti-VILIP3 antibody

VILIP3, NT (Visinin-like Protein 3, Calcium-binding Protein BDR-1, HLP2, Hippocalcin-like Protein 1, HPCAL1, BDR1) (Azide free) (HRP)

Cat. Num. AAA6505853

• Gene Names: HPCAL1; BDR1; HLP2; VILIP-3
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: VILIP3; Polyclonal Antibody; NT (Visinin-like Protein 3; Calcium-binding Protein BDR-1; HLP2; Hippocalcin-like Protein 1; HPCAL1; BDR1) (Azide free) (HRP); anti-VILIP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human VILIP3. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-VILIP3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-term region of human VILIP3 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of VILIP3 (arrow) using rabbit polyclonal VILIP3 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VILIP3 gene (Lane 2) (Origene Technologies).)

Western Blot (WB)

(The anti-VILIP3 Pab is used in Western blot to detect VILIP3 in mouse cerebellum tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-VILIP3 antibody

Antibodies; Neuroscience

References

1. Braunewell, K., et al., Dement Geriatr Cogn Disord 12(2):110-116 (2001). 2. Bernstein, H.G., et al., J Neurocytol 28(8):655-662 (1999). 3. Kobayashi, M., et al., Biochim. Biophys. Acta 1222(3):515-518 (1994). 4. Hidaka, H., et al., Neurosci. Res. 16(2):73-77 (1993). 5. Ivings, L., et al., Biochem. J. 363(Pt 3), 599-608 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 385298701
• NCBI GeneID: 3241
• NCBI Accession #: NP_001245286.1
• NCBI GenBank Nucleotide #: NM_001258357.1
• UniProt Accession #: P37235; Q969S5
• Molecular Weight: 22,313 Da
• NCBI Official Full Name: hippocalcin-like protein 1
• NCBI Official Synonym Full Names: hippocalcin-like 1
• NCBI Official Symbol: HPCAL1
• NCBI Official Synonym Symbols: BDR1; HLP2; VILIP-3
• NCBI Protein Information: hippocalcin-like protein 1; calcium-binding protein BDR-1; visinin-like protein 3
• UniProt Protein Name: Hippocalcin-like protein 1
• UniProt Gene Name: HPCAL1
• UniProt Synonym Gene Names: BDR1; VILIP-3
• UniProt Entry Name: HPCL1_HUMAN

NCBI Description

The protein encoded by this gene is a member of neuron-specific calcium-binding proteins family found in the retina and brain. It is highly similar to human hippocalcin protein and nearly identical to the rat and mouse hippocalcin like-1 proteins. It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation and may be of relevance for neuronal signalling in the central nervous system. Several alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

HPCAL1: May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Belongs to the recoverin family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 2p25.1

Molecular Function: protein binding; calcium ion binding

Biological Process: signal transduction