Rabbit VHL Polyclonal Antibody | anti-VHL antibody

Rabbit anti VHL

Cat. Num. AAA225176

• Gene Names: VHL; RCA1; VHL1; pVHL; HRCA1
• Reactivity: Human, Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Applications: Western Blot
• Purity: Purified whole serum by affinity chromatography.
• Synonyms: VHL; Polyclonal Antibody; Rabbit anti VHL; Vhl Antibody; anti-VHL antibody

• Host: Rabbit
• Reactivity: Human, Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified whole serum by affinity chromatography.
• Form/Format: Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
• Sequence Length: 213

• Applicable Applications for anti-VHL antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant human VHL
• Preperation: Antiserum to VHL was raised by repeated immunization of rabbits with highly purified antigen.
• Preparation and Storage: Store at -20 degree C.; Storage in frost-free freezers is not recommended. This product should be stored undiluted.; Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch

Western Blot (WB)

(Western blot analysis of cell lysates using Rabbit anti VHL antibody)

Related Product Information for anti-VHL antibody

Rabbit anti VHL antibody recognizes Von Hippel-Lindau disease tumor suppressor protein also known as Protein G7. VHL belongs to the VHL family of proteins and is involved in ubiquitination and proteosomal degradation via the von Hippel-Lindau ubiquitination complex.

NCBI and Uniprot Product Information

• NCBI GI #: 4033778
• NCBI GeneID: 7428
• UniProt Accession #: P40337; Q13599; Q6PDA9; B2RE45
• Molecular Weight: 18,532 Da
• NCBI Official Full Name: von Hippel-Lindau disease tumor suppressor
• NCBI Official Synonym Full Names: von Hippel-Lindau tumor suppressor
• NCBI Official Symbol: VHL
• NCBI Official Synonym Symbols: RCA1; VHL1; pVHL; HRCA1
• NCBI Protein Information: von Hippel-Lindau disease tumor suppressor
• UniProt Protein Name: von Hippel-Lindau disease tumor suppressor
• Protein Family: Von Hippel-Lindau disease tumor suppressor
• UniProt Gene Name: VHL

NCBI Description

Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2.