Goat DUSP6/MKP3 Polyclonal Antibody | anti-DUSP6 antibody

Goat anti-DUSP6/MKP3 Antibody

Cat. Num. AAA423072

• Gene Names: DUSP6; HH19; MKP3; PYST1
• Reactivity: Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: DUSP6/MKP3; Polyclonal Antibody; Goat anti-DUSP6/MKP3 Antibody; DUSP6; dual specificity phosphatase 6; MKP3; PYST1; MAP kinase phosphatase 3; dual specificity protein phosphatase 6; dual specificity protein phosphatase PYST1; mitogen-activated protein kinase phosphatase 3; serine/threonine specific protein phosphatase; DUSP6 / MKP3; anti-DUSP6 antibody

• Host: Goat
• Reactivity: Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: PSNQNVYQVDSLQST
• Sequence Length: 381

• Applicable Applications for anti-DUSP6 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Western Blot: Approx 26kDa band observed in Mouse and Rat Heart lysates and 40kDa plus 26kDa bands observed in Human and Pig Heart lysates (calculated MW of 42.3kDa according to NP_001937.2 and 26.5kDa according to NP_073143.2). Recommended concentration: 0.3-1ug/ml.
• Immunogen: Peptide with sequence C-PSNQNVYQVDSLQST, from the C Terminus of the protein sequence according to NP_001937.2; NP_073143.2.
• Epitope: C Terminus
• Note: This antibody is expected to recognize both reported isoforms (NP_001937.2; NP_073143.2).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of Human (A), (Mouse) and Rat (C) Heart lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

((0.3ug/ml) staining of Pig Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 42764683
• NCBI GeneID: 1848
• NCBI Accession #: NP_001937.2; NP_073143.2
• NCBI GenBank Nucleotide #: NM_001946.3
• Molecular Weight: 26,468 Da
• NCBI Official Full Name: dual specificity protein phosphatase 6 isoform a
• NCBI Official Synonym Full Names: dual specificity phosphatase 6
• NCBI Official Symbol: DUSP6
• NCBI Official Synonym Symbols: HH19; MKP3; PYST1
• NCBI Protein Information: dual specificity protein phosphatase 6
• UniProt Protein Name: Dual specificity protein phosphatase 6
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP6
• UniProt Synonym Gene Names: MKP3; PYST1; MAP kinase phosphatase 3; MKP-3
• UniProt Entry Name: DUS6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Mutations in this gene have been associated with congenital hypogonadotropic hypogonadism. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2014]

Uniprot Description

MKP-3: a non-receptor, dual-specificity phosphoprotein phosphatase. Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This protein inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two alternatively spliced isoforms have been described.

Protein type: EC 3.1.3.16; EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 12q22-q23

Cellular Component: cytoplasm; cytosol; nucleoplasm

Molecular Function: MAP kinase tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity

Biological Process: activation of MAPK activity; dorsal/ventral pattern formation; inactivation of MAPK activity; MAPKKK cascade; positive regulation of apoptosis; regulation of endodermal cell fate specification; regulation of fibroblast growth factor receptor signaling pathway; response to nitrosative stress

Disease: Hypogonadotropic Hypogonadism 19 With Or Without Anosmia