Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of VGF (K458) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)
Rabbit VGF Polyclonal Antibody | anti-VGF antibody
VGF (K458) Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
VGF; Polyclonal Antibody; VGF (K458) Polyclonal Antibody; Neurosecretory protein VGF; VGF nerve growth factor inducible; anti-VGF antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
VGF (K458) polyclonal antibody detects endogenous levels of VGF protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
615
Applicable Applications for anti-VGF antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 431-480 of Human VGF.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of VGF (K458) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of VGF (K458) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)
Related Product Information for anti-VGF antibody
Nerve growth factor (NGF) is a peptide that plays a key role in the differentiation and survival of neurons in the peripheral nervous system (PNS) and the central nervous system (CNS). VGF is a peptide synthesized and secreted by neurons and is upregulated by NGF in the PC12 cell line. VGF is widely expressed in both the PNS and CNS, but is especially abundant in the adult hypothalamus. VGF plays an essential role in how the brain regulates energy expenditure and body weight. Its expression is rapidly induced by injury, the circadian clock, and neuronal activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 67kDa
NCBI Official Full Name
neurosecretory protein VGF
UniProt Protein Name
Neurosecretory protein VGF
Protein Family
UniProt Gene Name
VGF
UniProt Synonym Gene Names
NERP-1; NERP-2
UniProt Entry Name
VGF_HUMAN
Uniprot Description
VGF: May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 7q22.1
Cellular Component: extracellular space; transport vesicle; cytoplasmic vesicle
Molecular Function: neuropeptide hormone activity; growth factor activity
Biological Process: response to dietary excess; generation of precursor metabolites and energy; response to cAMP; ovarian follicle development; sexual reproduction; insulin secretion; defense response to bacterium; response to cold; glucose homeostasis; response to insulin stimulus
Similar Products
Product Notes
The VGF vgf (Catalog #AAA3003663) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VGF (K458) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's VGF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the VGF vgf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VGF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
