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Western Blot (WB) (Western blot analysis of VGAT expression in mouse eye (A) whole cell lysates.)

Rabbit VGAT Polyclonal Antibody | anti-SLC32A1 antibody

Anti-VGAT Antibody

Gene Names
SLC32A1; VGAT; VIAAT
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
VGAT; Polyclonal Antibody; Anti-VGAT Antibody; VIAAT; Vesicular inhibitory amino acid transporter; GABA and glycine transporter; Solute carrier family 32 member 1; Vesicular GABA transporter; hVIAAT; anti-SLC32A1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of VGAT protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
525
Applicable Applications for anti-SLC32A1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1/500 - 1/2000; IHC: 1/50 - 1/200
Immunogen
Recombinant full length protein of human VGAT
Preparation and Storage
Ships on blue ice. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of VGAT expression in mouse eye (A) whole cell lysates.)

Western Blot (WB) (Western blot analysis of VGAT expression in mouse eye (A) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of VGAT staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of VGAT staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-SLC32A1 antibody
Rabbit polyclonal antibody to VGAT

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
vesicular inhibitory amino acid transporter
NCBI Official Synonym Full Names
solute carrier family 32 member 1
NCBI Official Symbol
SLC32A1
NCBI Official Synonym Symbols
VGAT; VIAAT
NCBI Protein Information
vesicular inhibitory amino acid transporter
UniProt Protein Name
Vesicular inhibitory amino acid transporter
UniProt Gene Name
SLC32A1
UniProt Synonym Gene Names
VGAT; VIAAT; hVIAAT
UniProt Entry Name
VIAAT_HUMAN

NCBI Description

The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II. [provided by RefSeq, Jul 2008]

Uniprot Description

SLC32A1: Involved in the uptake of GABA and glycine into the synaptic vesicles. Belongs to the amino acid/polyamine transporter 2 family.

Protein type: Membrane protein, integral; Transporter; Membrane protein, multi-pass; Transporter, SLC family

Chromosomal Location of Human Ortholog: 20q11.23

Cellular Component: dendrite; integral to membrane; neuron projection; plasma membrane; presynaptic active zone; synaptic vesicle membrane

Molecular Function: amino acid transmembrane transporter activity; gamma-aminobutyric acid:hydrogen symporter activity; glycine transmembrane transporter activity; hydrogen:vesicular amine antiporter activity

Biological Process: aging; amine transport; gamma-aminobutyric acid transport; glycine transport; hippocampus development; ion transport; neurotransmitter secretion

Research Articles on SLC32A1

Similar Products

Product Notes

The SLC32A1 slc32a1 (Catalog #AAA8245543) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-VGAT Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's VGAT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1/500 - 1/2000; IHC: 1/50 - 1/200. Researchers should empirically determine the suitability of the SLC32A1 slc32a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VGAT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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