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Western Blot (WB) (Western Blot: Sample: Human Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Human VAPA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Human Vesicle Associated Membrane Protein Associated Protein A (VAPA) Polyclonal Antibody | anti-VAPA antibody

Polyclonal Antibody to Vesicle Associated Membrane Protein Associated Protein A (VAPA)

Gene Names
VAPA; VAP-A; VAP33; VAP-33; hVAP-33
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Vesicle Associated Membrane Protein Associated Protein A (VAPA); Polyclonal Antibody; Polyclonal Antibody to Vesicle Associated Membrane Protein Associated Protein A (VAPA); anti-VAPA antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against VAPA. It has been selected for its ability to recognize VAPA in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-MASASGAMAK HEQILVLDPP TDLKFKGPFT DVVTTNLKLR NPSDRKVCFK VKTTAPRRYC VRPNSGIIDP GSTVTVSVML QPFDYDPNEK SKHKFMVQTI FAPPNTSDME AVWKEAKPDE LMDSKLRCVF EMPNENDKLN DMEPSKAVPL NASKQDGPMP KPHSVSLNDT ETRKLMEECK RLQGEMMKLS EENRHLRDEG LRLRKVAHSD KPGSTSTASF RDNVTSPLPS LLVVIAAIFI GFFLGKFIL
Sequence Length
249
Applicable Applications for anti-VAPA antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant VAPA (Met1~Leu249) expressed in E.coli.
Cross Reactivity
Human, Mouse, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2076901
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Human Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Human VAPA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Human Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Human VAPA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Stomach tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded Stomach tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,614 Da
NCBI Official Full Name
vesicle-associated membrane protein-associated protein A isoform 1
NCBI Official Synonym Full Names
VAMP associated protein A
NCBI Official Symbol
VAPA
NCBI Official Synonym Symbols
VAP-A; VAP33; VAP-33; hVAP-33
NCBI Protein Information
vesicle-associated membrane protein-associated protein A
UniProt Protein Name
Vesicle-associated membrane protein-associated protein A
UniProt Gene Name
VAPA
UniProt Synonym Gene Names
VAP33; VAMP-A; VAMP-associated protein A; VAP-A; VAP-33

NCBI Description

The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

VAPA: May play a role in vesicle trafficking. Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 18p11.22

Cellular Component: bicellular tight junction; endoplasmic reticulum; endoplasmic reticulum exit site; endoplasmic reticulum membrane; Golgi membrane; integral component of membrane; microtubule cytoskeleton; nuclear membrane; perinuclear region of cytoplasm; plasma membrane; vesicle

Molecular Function: cadherin binding; FFAT motif binding; microtubule binding; protein binding; protein domain specific binding; protein heterodimerization activity; signal transducer activity

Biological Process: cell death; COPII-coated vesicle budding; endoplasmic reticulum organization; ER to Golgi vesicle-mediated transport; neuron projection development; neutrophil degranulation; positive regulation by host of viral release from host cell; positive regulation of I-kappaB kinase/NF-kappaB signaling; protein localization to endoplasmic reticulum; signal transduction; sphingolipid biosynthetic process

Research Articles on VAPA

Similar Products

Product Notes

The VAPA vapa (Catalog #AAA2001849) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Vesicle Associated Membrane Protein Associated Protein A (VAPA) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Vesicle Associated Membrane Protein Associated Protein A (VAPA) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the VAPA vapa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-MASASG AMAK HEQILVLDPP TDLKFKGPFT DVVTTNLKLR NPSDRKVCFK VKTTAPRRYC VRPNSGIIDP GSTVTVSVML QPFDYDPNEK SKHKFMVQTI FAPPNTSDME AVWKEAKPDE LMDSKLRCVF EMPNENDKLN DMEPSKAVPL NASKQDGPMP KPHSVSLNDT ETRKLMEECK RLQGEMMKLS EENRHLRDEG LRLRKVAHSD KPGSTSTASF RDNVTSPLPS LLVVIAAIFI GFFLGKFIL. It is sometimes possible for the material contained within the vial of "Vesicle Associated Membrane Protein Associated Protein A (VAPA), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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