Western Blot (WB)
(Anti- Versican Picoband antibody, MBS178301, Western blottingAll lanes: Anti Versican (MBS178301) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: HEPA Whole Cell Lysate at 40ugPredicted bind size: 373KDObserved bind size: 373KD )
Versican Polyclonal Antibody | anti-VCAN antibody
Anti-Versican Antibody
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
Western Blot (WB)
(Anti- Versican Picoband antibody, MBS178301, Western blottingAll lanes: Anti Versican (MBS178301) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: HEPA Whole Cell Lysate at 40ugPredicted bind size: 373KDObserved bind size: 373KD )
Western Blot (WB)
(Anti- Versican Picoband antibody, MBS178301, Western blottingAll lanes: Anti Versican (MBS178301) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: HEPA Whole Cell Lysate at 40ugPredicted bind size: 373KDObserved bind size: 373KD )
Immunohistochemistry (IHC)
(Anti- Versican Picoband antibody, MBS178301, IHC(P)IHC(P): Human Glioma Tissue )
Immunohistochemistry (IHC)
(Anti- Versican Picoband antibody, MBS178301, IHC(P)IHC(P): Human Glioma Tissue )
Background: Versican (VCAN), also known as CSPG2, is a large extracellular matrix proteoglycan that is present in a variety of human tissues. Versican is a large chondroitin sulfate proteoglycan with an apparent molecular mass of more than 1000kDa. The Versican gene is mapped on 5q14.2-q14.3. The human versican gene contains 15 exons spanning more than 90 kb. The distribution of versican by using affinity-purified polyclonal antibodies that recognize the core protein of the prominent versican splice variants V0 and V1. Versican staining was noted in the central and peripheral nervous system, in the basal layer of the epidermis, and on the luminal surface of some glandular epithelia. Biochemical purification of LLC-conditioned medium led to identification of the extracellular matrix proteoglycan versican, which is upregulated in many human tumors including lung cancer, as a macrophage activator that acts through TLR2 and its coreceptors TLR6 and CD14. By activating TLR2:TLR6 complexes and inducing TNF-alpha secretion by myeloid cells, versican strongly enhances LLC metastatic growth.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
Uniprot Description
CSPG2: May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid. Defects in VCAN are the cause of Wagner syndrome type 1 (WGN1). WGN is a dominantly inherited vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a preretinal avascular membrane. Other optical features include progressive chorioretinal atrophy, perivascular sheating, subcapsular cataract and myopia. Systemic manifestations are absent in WGN. Belongs to the aggrecan/versican proteoglycan family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Cell adhesion; Secreted
Chromosomal Location of Human Ortholog: 5q14.3
Cellular Component: extracellular matrix; extracellular region; extracellular space; Golgi lumen; intracellular membrane-bound organelle; lysosomal lumen; membrane; proteinaceous extracellular matrix
Molecular Function: calcium ion binding; carbohydrate binding; extracellular matrix structural constituent; glycosaminoglycan binding; hyaluronic acid binding; protein binding
Biological Process: cell adhesion; cell recognition; central nervous system development; chondroitin sulfate biosynthetic process; chondroitin sulfate catabolic process; dermatan sulfate biosynthetic process; extracellular matrix organization and biogenesis; glial cell migration; glycosaminoglycan metabolic process; multicellular organismal development; osteoblast differentiation; skeletal development
Disease: Wagner Vitreoretinopathy
Research Articles on VCAN
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Product Notes
The VCAN vcan (Catalog #AAA178301) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Versican Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Versican can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot Concentration: 0.1-0.5ug/ml Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml. Researchers should empirically determine the suitability of the VCAN vcan for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Versican, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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