Rabbit VEGFR3 Polyclonal Antibody | anti-FLT4 antibody

Rabbit Anti-Mouse VEGFR3

Cat. Num. AAA625970

• Gene Names: FLT4; PCL; FLT41; LMPH1A; VEGFR3
• Applications: ELISA, Immunohistochemistry
• Purity: Purified by immunoaffinity chromatography
• Synonyms: VEGFR3; Polyclonal Antibody; Rabbit Anti-Mouse VEGFR3; Anti -VEGFR3 (Vascular Endothelial Growth Factor Receptor 3; VEGFR-3; Fms-like Tyrosine Kinase 4; FLT-4; Tyrosine-protein Kinase Receptor FLT4; FLT4); anti-FLT4 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG1
• Specificity: Recognizes mouse VEGFR3.
• Purity/Purification: Purified by immunoaffinity chromatography
• Form/Format: Supplied as a lyophilized powder from PBS, 0.1% BSA.Reconstitute with 100ul sterile PBS.
• Concentration: ~1mg/ml (afterreconstitution) (varies by lot)

• Applicable Applications for anti-FLT4 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:10,000-1:100,000; 50-100ng of control peptide/well; Immunohistochemistry: 2-20ug/ml in formaldehyde fixed tissues
• Immunogen: Synthetic peptide corresponding to 20aa near the C-terminal,cytoplasmic domain of mouse VEGFR3, conjugated to KLH.Species Sequence Homology: rat; 85%, human; 73%
• Control Peptide: F4601-03: Flt4, Mouse, Control Peptide (Fms-like Tyrosine Kinase 4)
• Grade: Affinity Purified
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-FLT4 antibody

Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). Study of tumor angiogenesis has led to the identification of several proteins including basic fibroblast growth factor (bFGF) and vascular endothelial growth factor. VEGF acts by interacting with a family of largely endothelial-specific receptor tyrosine kinases that includes VEGFR1 (flt-1/flk-2), VEGFR2 (flk- 1/KDR), and VEGFR3/Flt-4. Disruption of VEGFRs interferes with differentiation of endothelial cells and it is lethal for the embryo. VEGFR3 (mouse 1363aa and human 1298aa), a receptor protein tyrosine kinase, is a receptor for VEGF-C. Mouse VEGFR3 protein topology: 1-24aa signal peptide, 25-1363 mature protein (25-775 EC domain, 776-797aa TM domain, 798-1363aa CP domain). VEGFR3 is type 1 membrane protein. It is expressed in adult lung, and liver, and in fetal liver, brain, intestine, and placenta.

Product Categories/Family for anti-FLT4 antibody

Antibodies; Abs to Receptors

References

Cardiac Mouse Lymphatics: Developmental and Anatomical Update. ALEKSANDRA FLAHT-ZABOST, GRZEGORZ GULA, BOGDAN CISZEK, ELZBIETA CZARNOWSKA, EWA JANKOWSKA-STEIFER, MARIA MADEJ, JUSTYNA NIDERLA-BIELINSKA, DOROTA RADOMSKA-LESNIEWSKA, AND ANNA RATAJSKA (2014)

NCBI and Uniprot Product Information

• NCBI GI #: 104294888
• NCBI GeneID: 2324
• NCBI Accession #: NP_891555.2
• NCBI GenBank Nucleotide #: NM_182925.4
• UniProt Accession #: P35916; Q16067; Q86W07; Q59F24; Q86W08; AAB23636
• NCBI Official Full Name: vascular endothelial growth factor receptor 3 isoform 1
• NCBI Official Synonym Full Names: fms-related tyrosine kinase 4
• NCBI Official Symbol: FLT4
• NCBI Official Synonym Symbols: PCL; FLT41; LMPH1A; VEGFR3
• NCBI Protein Information: vascular endothelial growth factor receptor 3; FLT-4; VEGFR-3; OTTHUMP00000161539; OTTHUMP00000223511; OTTHUMP00000223512; soluble VEGFR3 variant 1; soluble VEGFR3 variant 2; soluble VEGFR3 variant 3; fms-like tyrosine kinase 4; tyrosine-protein kinase receptor FLT4
• UniProt Protein Name: Vascular endothelial growth factor receptor 3
• Protein Family: Vascular endothelial growth factor receptor
• UniProt Gene Name: FLT4
• UniProt Entry Name: VGFR3_HUMAN

NCBI Description

This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA. [provided by RefSeq]

Uniprot Description

VEGFR3: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades; isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3- kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr- 185', and of AKT1 at 'Ser-473'. Interacts with VEGFC and VEGFD. Monomer in the absence of bound VEGFC or VEGFD. Homodimer in the presence of bound VEGFC or VEGFD. Can also form a heterodimer with KDR. Interacts with PTPN14; the interaction is enhanced by stimulation with VEGFC. Interacts with CRK, GRB2, PTK2/FAK1, SHC1, PIK3R1 and PTPN11/SHP- 2. Identified in a complex with SRC and ITGB1. Detected in endothelial cells. Widely expressed. Detected in fetal spleen, lung and brain. Detected in adult liver, muscle, thymus, placenta, lung, testis, ovary, prostate, heart, and kidney. Present in an inactive conformation in the absence of bound ligand. Binding of VEGFC or VEGFD leads to dimerization and activation by autophosphorylation on tyrosine residues. Inhibited by MAZ51. Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.10.1; Protein kinase, TK; Protein kinase, tyrosine (receptor); Kinase, protein; Membrane protein, integral; TK group; VEGFR family

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: integral to plasma membrane; cytoplasm; extracellular region; plasma membrane; nucleus; receptor complex

Molecular Function: vascular endothelial growth factor receptor activity; protein binding; growth factor binding; transmembrane receptor protein tyrosine kinase activity; protein phosphatase binding; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; positive regulation of JNK cascade; vasculature development; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; lymphangiogenesis; blood vessel morphogenesis; positive regulation of endothelial cell proliferation; sprouting angiogenesis; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; lymph vessel development; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Hemangioma, Capillary Infantile; Lymphedema, Hereditary, Ia