Rabbit anti-Human VEGFR-2 Polyclonal Antibody | anti-VEGFR-2 antibody

VEGFR-2, Phosphorylated (Tyr996) (Vascular Endothelial Growth Factor Receptor 2, VEGFR-2, Kinase Insert Domain Receptor, Protein-tyrosine Kinase Receptor Flk-1, Fetal Liver Kinase 1, FLK-1, CD309 antigen, KDR, FLK1) (FITC)

Cat. Num. AAA6505610

• Gene Names: KDR; FLK1; CD309; VEGFR; VEGFR2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: VEGFR-2; Polyclonal Antibody; Phosphorylated (Tyr996) (Vascular Endothelial Growth Factor Receptor 2; Kinase Insert Domain Receptor; Protein-tyrosine Kinase Receptor Flk-1; Fetal Liver Kinase 1; FLK-1; CD309 antigen; KDR; FLK1) (FITC); anti-VEGFR-2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KDR when phosphorylated at Tyr996.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-VEGFR-2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr996 of human KDR/FLK1 (KLH).
• Conjugate: FITC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Anti-Phospho-KDR/FLK1-Y996 Pab is used in Western blot to detect Phospho-KDR/FLK1-Y996 in HMVEC cell line lysate. Endothelial cells were stimulated with 50ug/ml VEGF for 5min; 20ug lysate from HMVEC was loaded onto an 8% gel; for Western blot, membranes were incubated O/N with Phospho-KDR/FLK1-Y996 Antibody (AP3107a)diluted to 1:500 in 1% Milk/TBST. Data and Protocol kindly provided by Dr. Weis from Cheresh Lab, UCSD.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-VEGFR-2 antibody

Antibodies; Receptors; Tyrosine Kinase

References

1. Le Boeuf, F., et al., J. Biol. Chem. 279(37):39175-39185 (2004). 2. Lee, Y.K., et al., Blood 104(3):788-794 (2004). 3. Sulpice, E., et al., Eur. J. Biochem. 271(16):3310-3318 (2004). 4. Murdaca, J., et al., J. Biol. Chem. 279(25):26754-26761 (2004). 5. List, A.F., et al., Exp. Hematol. 32(6):526-535 (2004). 6. Ina M. Wittko, et al. J. Neurosci. 2009; 29:8704-8714.

NCBI and Uniprot Product Information

• NCBI GI #: 11321597
• NCBI GeneID: 3791
• NCBI Accession #: NP_002244
• NCBI GenBank Nucleotide #: NM_002253.3
• UniProt Accession #: P35968; O60723; Q14178; A2RRS0; B5A925; C5IFA0
• Molecular Weight: 110.5kDa (987aa) 100-150KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: vascular endothelial growth factor receptor 2
• NCBI Official Synonym Full Names: kinase insert domain receptor
• NCBI Official Symbol: KDR
• NCBI Official Synonym Symbols: FLK1; CD309; VEGFR; VEGFR2
• NCBI Protein Information: vascular endothelial growth factor receptor 2
• UniProt Protein Name: Vascular endothelial growth factor receptor 2
• UniProt Gene Name: KDR
• UniProt Synonym Gene Names: FLK1; VEGFR2; VEGFR-2; FLK-1; KDR
• UniProt Entry Name: VGFR2_HUMAN

NCBI Description

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]

Uniprot Description

VEGFR2: a receptor tyrosine kinase of the VEGFR family. High affinity receptor for VEGF and VEGF-C. Ligand binding induces autophosphorylation and activation. Activated receptor recruits proteins including Shc, GRB2, PI3K, Nck, SHP-1 and SHP-2. Plays a key role in vascular development and regulation of vascular permeability.

Protein type: Membrane protein, integral; Kinase, protein; Protein kinase, tyrosine (receptor); Protein kinase, TK; EC 2.7.10.1; TK group; VEGFR family

Chromosomal Location of Human Ortholog: 4q11-q12

Cellular Component: Golgi apparatus; integral to plasma membrane; endoplasmic reticulum; early endosome; cytoplasmic membrane-bound vesicle; extracellular region; plasma membrane; cell junction; nucleus; endosome; external side of plasma membrane; lipid raft

Molecular Function: integrin binding; vascular endothelial growth factor receptor activity; protein binding; growth factor binding; protein-tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; Hsp90 protein binding; ATP binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of positive chemotaxis; peptidyl-tyrosine phosphorylation; viral reproduction; protein amino acid autophosphorylation; cell maturation; calcium ion homeostasis; regulation of cell shape; positive regulation of focal adhesion formation; ovarian follicle development; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; angiogenesis; vasculogenesis; endothelial cell differentiation; cell fate commitment; embryonic hemopoiesis; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of angiogenesis; cell migration during sprouting angiogenesis; branching morphogenesis of a tube; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; lymph vessel development; alveolus development; surfactant homeostasis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of cell migration

Disease: Hemangioma, Capillary Infantile