Rabbit VEGFB Polyclonal Antibody | anti-VEGFB antibody

VEGFB (R125) Polyclonal Antibody

Cat. Num. AAA3001175

• Gene Names: VEGFB; VRF; VEGFL
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: VEGFB; Polyclonal Antibody; VEGFB (R125) Polyclonal Antibody; Vascular endothelial growth factor B; VEGF-B; VEGF-related factor; VRF; anti-VEGFB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: VEGFB (R125) polyclonal antibody detects endogenous levels of VEGFB protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 188

• Applicable Applications for anti-VEGFB antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human VEGFB.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of VEGFB (R125) polyclonal antibody at 1:500 dilutionLane1:K562 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:The Heart tissue lysate of Rat (40ug) Lane4:The Heart tissue lysate of Mouse (40ug))

Related Product Information for anti-VEGFB antibody

The onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include fibroblast growth factor (FGF), platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). VEGF is a dimeric glycoprotein with struc- tural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo. Two additional proteins des- ignated VEGF-B and VEGF-C share a significant degree of homology withVEGF. VEGF-B is abundantly expressed in heart and skeletal muscle and is frequently co-expressed with VEGF.

NCBI and Uniprot Product Information

• NCBI GI #: 344179103
• NCBI GeneID: 7423
• NCBI Accession #: NP_001230662.1
• NCBI GenBank Nucleotide #: NP_001230662.1
• UniProt Accession #: P49765
• Molecular Weight: ~ 22kDa
• NCBI Official Full Name: vascular endothelial growth factor B isoform VEGFB-167
• NCBI Official Synonym Full Names: vascular endothelial growth factor B
• NCBI Official Symbol: VEGFB
• NCBI Official Synonym Symbols: VRF; VEGFL
• NCBI Protein Information: vascular endothelial growth factor B
• UniProt Protein Name: Vascular endothelial growth factor B
• Protein Family: Vascular endothelial growth factor
• UniProt Gene Name: VEGFB
• UniProt Synonym Gene Names: VRF; VEGF-B; VRF
• UniProt Entry Name: VEGFB_HUMAN

NCBI Description

This gene encodes a member of the PDGF (platelet-derived growth factor)/VEGF (vascular endothelial growth factor) family. The VEGF family members regulate the formation of blood vessels and are involved in endothelial cell physiology. This member is a ligand for VEGFR-1 (vascular endothelial growth factor receptor 1) and NRP-1 (neuropilin-1). Studies in mice showed that this gene was co-expressed with nuclear-encoded mitochondrial genes and the encoded protein specifically controlled endothelial uptake of fatty acids. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Sep 2011]

Uniprot Description

VEGFB: Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. Homodimer; disulfide-linked. Can also form heterodimer with VEGF. Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Cytokine; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: heparin binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; vascular endothelial growth factor receptor 1 binding; chemoattractant activity

Biological Process: response to drug; protein amino acid O-linked glycosylation; platelet activation; positive regulation of vascular permeability; positive regulation of vascular endothelial growth factor receptor signaling pathway; induction of positive chemotaxis; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; platelet degranulation; positive chemotaxis; positive regulation of cell division; positive regulation of endothelial cell proliferation; angiogenesis; negative regulation of neuron apoptosis; blood coagulation; vascular endothelial growth factor receptor signaling pathway; cardiac muscle contraction; negative regulation of apoptosis