Immunofluorescence (IF)/Immunocytochemistry (ICC)
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod)
Rabbit VEGFB Polyclonal Antibody | anti-VEGFB antibody
VEGFB Antibody
Predicted: Pig, Bovine, Horse, Sheep
Predicted: Pig, Bovine, Horse, Sheep
IF/ICC: 1:100-1:500
ELISA (Peptide): 1:20000-1:40000
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(At 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(At 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Western Blot (WB)
(Western blot analysis of extracts from 293t, using VEGFB Antibody. The lane on the left was treated with blocking peptide.Observed bands: 17 kDa.)
Western Blot (WB)
(Western blot analysis of extracts from 293t, using VEGFB Antibody. The lane on the left was treated with blocking peptide.Observed bands: 17 kDa.)
Western Blot (WB)
(Western blot analysis of extracts from MCF7, using VEGFB Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from MCF7, using VEGFB Antibody. The lane on the left was treated with blocking peptide.)
Post Translational Modifications: VEGF-B186 is O-glycosylated.
Subunit Structure: Homodimer; disulfide-linked. Can also form heterodimer with VEGF.
Similarity: Belongs to the PDGF/VEGF growth factor family.
NCBI and Uniprot Product Information
Predicted: 22 kDa
NCBI Description
This gene encodes a member of the PDGF (platelet-derived growth factor)/VEGF (vascular endothelial growth factor) family. The VEGF family members regulate the formation of blood vessels and are involved in endothelial cell physiology. This member is a ligand for VEGFR-1 (vascular endothelial growth factor receptor 1) and NRP-1 (neuropilin-1). Studies in mice showed that this gene was co-expressed with nuclear-encoded mitochondrial genes and the encoded protein specifically controlled endothelial uptake of fatty acids. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Sep 2011]
Uniprot Description
VEGFB: Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. Homodimer; disulfide-linked. Can also form heterodimer with VEGF. Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell cycle regulation; Cytokine; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: extracellular space; membrane; extracellular region
Molecular Function: heparin binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; vascular endothelial growth factor receptor 1 binding; chemoattractant activity
Biological Process: response to drug; protein amino acid O-linked glycosylation; platelet activation; positive regulation of vascular permeability; positive regulation of vascular endothelial growth factor receptor signaling pathway; induction of positive chemotaxis; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; platelet degranulation; positive chemotaxis; positive regulation of cell division; positive regulation of endothelial cell proliferation; angiogenesis; negative regulation of neuron apoptosis; blood coagulation; vascular endothelial growth factor receptor signaling pathway; cardiac muscle contraction; negative regulation of apoptosis
Research Articles on VEGFB
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Product Notes
The VEGFB vegfb (Catalog #AAA9610381) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VEGFB Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's VEGFB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA). WB: 1:500-1:2000 IF/ICC: 1:100-1:500 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the VEGFB vegfb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VEGFB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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