Rabbit Ephrin-A3 Polyclonal Antibody | anti-A3 antibody

Ephrin-A3 (E196) Polyclonal Antibody

Cat. Num. AAA3002047

• Gene Names: EFNA3; EFL2; EPLG3; LERK3; Ehk1-L
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Ephrin-A3; Polyclonal Antibody; Ephrin-A3 (E196) Polyclonal Antibody; Ephrin A3; EFL-2; EHK1 ligand; EHK1-L; EPH-related receptor tyrosine kinase ligand 3; LERK-3; EFNA3; EFL2; EPLG3; LERK3; anti-A3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Ephrin-A3 (E196) polyclonal antibody detects endogenous levels of Ephrin-A3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 238

• Applicable Applications for anti-A3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 180-230 of Human Ephrin-A3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Ephrin-A3 (E196) pAb at 1:500 dillutionLane1:Hct116 whole cell lysateLane2:SK-OVCAR3 whole cell lysateLane3:The Brain tissue lysate of MouseLane4:The Brain tissue lysate of Rat)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Ephrin-A3 (E196) pAb in paraffin-embedded human brain tissue.)

Related Product Information for anti-A3 antibody

The Eph subfamily represents the largest group of receptor protein kinases identified to date. There is increasing evidence that they are involved in central nervous system function and in development. Ligands for Eph receptors include ephrin-A1 (LERK-1/B61), identified as a ligand for the EphA2 (Eck) receptor; ephrin-A2 (ELF-1), identified as a ligand for the EphA3 and EphA4 (Sek) receptors; ephrin-A3 (LERK-3), identified as a ligand for EphA5 (Ehk1) and EphA3 (Hek) receptors; ephrin-A4 (LERK-4), identified as a ligand for the EphA3 receptor; ephrin-A5 (AL-1), identified as a ligand for EphA5 (REK7); ephrin-B1 (LERK-2), identified as a ligand for the EphB1 (Elk) and EphB2 (Cek5) receptors; ephrin-B2 (LERK-5), identified as a ligand for the EphB1, EphB3 (Cek10) and EphB2 receptors; and ephrin-B3 (LERK-8), identified as a ligand for EphB1.

NCBI and Uniprot Product Information

• NCBI GI #: 4826708
• NCBI GeneID: 1944
• NCBI Accession #: NP_004943.1
• NCBI GenBank Nucleotide #: NP_004943.1
• UniProt Accession #: P52797
• Molecular Weight: ~ 38kDa
• NCBI Official Full Name: ephrin-A3
• NCBI Official Synonym Full Names: ephrin A3
• NCBI Official Symbol: EFNA3
• NCBI Official Synonym Symbols: EFL2; EPLG3; LERK3; Ehk1-L
• NCBI Protein Information: ephrin-A3
• UniProt Protein Name: Ephrin-A3
• Protein Family: Ephrin
• UniProt Gene Name: EFNA3
• UniProt Synonym Gene Names: EFL2; EPLG3; LERK3; EHK1-L; LERK-3
• UniProt Entry Name: EFNA3_HUMAN

NCBI Description

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin. [provided by RefSeq, Jul 2008]

Uniprot Description

EFNA3: Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Belongs to the ephrin family.

Protein type: Ligand, receptor tyrosine kinase; Membrane protein, GPI anchor; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: ephrin receptor binding; transmembrane-ephrin receptor activity

Biological Process: axon guidance; cell-cell signaling; ephrin receptor signaling pathway