Testing Data
(Staining in FFPE human angiosarcoma using MBS618634.)
Rabbit VEGF R2 Polyclonal Antibody
VEGF R2 (FLK1/KDR)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
VEGF R2; Polyclonal Antibody; VEGF R2 (FLK1/KDR); Anti -VEGF R2 (FLK1/KDR); anti-VEGF R2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
We have not characterized its specifity to various forms. To our knowledge, it has only been shown to recognize the native form (180-190kD protein) known as Flk-1 / KDR / VEGFR 2 (Vascular Endothelial Growth Factor Receptor 2) though it was raised against the precursor form.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.05% sodium azide.
Applicable Applications for anti-VEGF R2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Dilution: Immunofluorescence: 1:50-1:100
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:50-1:100 (ABC)
Dilution: Immunofluorescence: 1:50-1:100
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:50-1:100 (ABC)
Immunogen
Synthetic peptide derived from the C-terminus of the precursor form of the mouse VEGF R2
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Staining in FFPE human angiosarcoma using MBS618634.)
Testing Data
(Staining in FFPE human angiosarcoma using MBS618634.)
Related Product Information for anti-VEGF R2 antibody
VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity.
Product Categories/Family for anti-VEGF R2 antibody
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Product Notes
The VEGF R2 (Catalog #AAA618634) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VEGF R2 (FLK1/KDR) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's VEGF R2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Dilution: Immunofluorescence: 1:50-1:100 Western Blot: 1:500-1:2000 Immunohistochemistry: 1:50-1:100 (ABC). Researchers should empirically determine the suitability of the VEGF R2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VEGF R2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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