Rabbit VE Cadherin Polyclonal Antibody | anti-CDH5 antibody

Polyclonal Anti-CDH5 Antibody; Anti-VE Cadherin antibody

Cat. Num. AAA176401

• Gene Names: CDH5; 7B4; CD144
• Reactivity: Human, Rat; Predicted: Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: VE Cadherin; Polyclonal Antibody; Polyclonal Anti-CDH5 Antibody; Anti-VE Cadherin antibody; Cadherin-5; cadherin 5; type 2(vascular endothelium); 7B 4 antibody; 7B4 antibody; 7B4 antigen antibody; CADH5_HUMAN antibody; Cadherin 5 antibody; Cadherin 5 type 2 antibody; Cadherin 5; type 2(vascular endothelium) antibody; type 2; VE cadherin(vascular epithelium) antibody; cadherin; vascular endothelial antibody; vascular endothelial; 1 antibody; Cadherin-5 antibody; Cadherin5 antibody; CD 144 antibody; CD144 antibody; CD144 antigen antibody; CDH 5 antibody; CDH5 antibody; CDH5 protein antibody; Endothelial specific cadherin antibody; FLJ17376 antibody; OTTHUMP00000174777 antibody; Vascular endothelial cadherin antibody; Vascular epithelium cadherin antibody; VE Cad antibody; VE-cadherin antibody; VEC antibody; anti-CDH5 antibody

• Host: Rabbit
• Reactivity: Human, Rat; Predicted: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 784

• Applicable Applications for anti-CDH5 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot:; Concentration: 0.1-0.5ug/ml; Tested Species: Human; Predicted Species: N/A; Antigen retrieval: N/A; ; Immunohistochemistry (Paraffin-embedded Section):; Concentration: 0.5-1ug/ml; Tested Species: Human, Rat; Predicted Species: Mouse; Antigen retrieval: By Heat; ; Tested Species: In-house tested species with positive results; Predicted Species: Species predicted to be fit for the product based on sequence similarities.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20mins is required for the staining of formalin/paraffin sections.; Other applications have not been tested, Optimal dilutions should be determined by end users.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CDH5(766-784aa RFKMLAELYGSDPREELLY), different from the related mouse and rat sequences by three amino acids.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg Thimerosal, 0.05mg NaN₃.
• Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-VE Cadherin antibody, MBS176401, Western blottingAll lanes: Anti VE Cadherin (MBS176401) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 87KDObserved bind size: 130KD)

Immunohistochemistry (IHC)

(Anti-VE Cadherin antibody, MBS176401, IHC(P)IHC(P): Human Lung Cancer Tissue )

Immunohistochemistry (IHC)

(Anti-VE Cadherin antibody, MBS176401, IHC(P)IHC(P): Rat Brain Tissue )

Related Product Information for anti-CDH5 antibody

CDH5(Cadherin 5), also known as VE-cadherin, is a type of cadherin. It is encoded by the human gene CDH5. Kremmidiotis et al.(1998) mapped the human CDH5 gene to 16q22.1 using somatic cell hybrid panels. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Therefore it was concluded that VE-cadherin serves the purpose of maintaining newly formed vessels.

NCBI and Uniprot Product Information

• NCBI GI #: 322510142
• NCBI GeneID: 1003
• UniProt Accession #: P33151; Q4VAI5; Q4VAI6
• NCBI Official Full Name: Cadherin-5
• NCBI Official Synonym Full Names: cadherin 5, type 2 (vascular endothelium)
• NCBI Official Symbol: CDH5
• NCBI Official Synonym Symbols: 7B4; CD144
• NCBI Protein Information: cadherin-5; 7B4 antigen; VE-cadherin; cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin; cadherin 5, type 2, VE-cadherin (vascular epithelium)
• UniProt Protein Name: Cadherin-5
• Protein Family: Cadherin
• UniProt Gene Name: CDH5
• UniProt Synonym Gene Names: VE-cadherin
• UniProt Entry Name: CADH5_HUMAN

NCBI Description

This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: adherens junction; tight junction; membrane; integral to membrane; plasma membrane; intercellular junction; cell junction

Molecular Function: protein binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; receptor binding

Biological Process: intercellular junction assembly and maintenance; negative regulation of cell proliferation; vascular endothelial growth factor receptor signaling pathway; homophilic cell adhesion; blood vessel maturation