Rabbit VCL / Vinculin Polyclonal Antibody | anti-VCL antibody

Rabbit Polyclonal (IgG) to Human VCL / Vinculin

Cat. Num. AAA246738

• Gene Names: VCL; MV; MVCL; CMD1W; CMH15; HEL114
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: VCL / Vinculin; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human VCL / Vinculin; Anti-VCL / Vinculin Antibody (aa786-835) IHC-plus; VCL; CMD1W; CMH15; Metavinculin; Vinculin; MVCL; Human VCL; anti-VCL antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Vinculin (Ab-821) Antibody detects endogenous levels of total Vinculin protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1134

• Applicable Applications for anti-VCL antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000), IF (1:100 - 1:500), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human Vinculin (aa786-835).
• Immunogen: VCL / Vinculin antibody was raised against synthetic peptide from human Vinculin (aa786-835).
• Antigen Modification: aa786-835
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-Vinculin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-Vinculin antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of A549 cells, using Vinculin Antibody. The picture on the right is treated with the synthesized peptide.)

Western Blot (WB)

(Western blot of extracts from HeLa cells, treated with forskolin 40 nM 30 minutes, using Vinculin Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-VCL antibody

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. Human vinculin protein exhibits a greater than 95% sequence identity to the chicken vinculin protein.

Product Categories/Family for anti-VCL antibody

Family: Cytoskeleton

NCBI and Uniprot Product Information

• NCBI GI #: 7669550
• NCBI GeneID: 7414
• NCBI Accession #: NP_054706.1
• NCBI GenBank Nucleotide #: NM_014000.2
• UniProt Accession #: P18206; Q16450; Q5SWX2; Q7Z3B8; Q8IXU7
• Molecular Weight: 24,904 Da
• NCBI Official Full Name: vinculin isoform meta-VCL
• NCBI Official Synonym Full Names: vinculin
• NCBI Official Symbol: VCL
• NCBI Official Synonym Symbols: MV; MVCL; CMD1W; CMH15; HEL114
• NCBI Protein Information: vinculin; metavinculin; epididymis luminal protein 114
• UniProt Protein Name: Vinculin
• UniProt Gene Name: VCL
• UniProt Synonym Gene Names: MV
• UniProt Entry Name: VINC_HUMAN

NCBI Description

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Vinculin: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: protein complex; costamere; focal adhesion; extracellular region; fascia adherens; intercellular junction; cytosol; cell-matrix junction; actin cytoskeleton; cytoskeleton; adherens junction; cell-cell adherens junction; plasma membrane; vesicle

Molecular Function: protein binding; cadherin binding; beta-catenin binding; actin binding; structural molecule activity; alpha-catenin binding

Biological Process: lamellipodium biogenesis; platelet activation; apical junction assembly; platelet degranulation; muscle contraction; axon extension; cell-matrix adhesion; morphogenesis of an epithelium; cell adhesion; blood coagulation; cell motility; negative regulation of cell migration

Disease: Cardiomyopathy, Dilated, 1w