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Western Blot (WB) (Western blot analysis of VAMP1/2/3 expression in HepG2 (A), mouse brain (B) whole cell lysates.)

Rabbit VAMP1/2/3 Polyclonal Antibody | anti-VAMP1 antibody

Anti-VAMP1/2/3 Antibody

Gene Names
VAMP1; SYB1; SPAX1; VAMP-1
Reactivity
Human, Mouse, Rat, Bovine
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
VAMP1/2/3; Polyclonal Antibody; Anti-VAMP1/2/3 Antibody; VAMP1; SYB1; Vesicle-associated membrane protein 1; VAMP-1; Synaptobrevin-1; VAMP2; SYB2; Vesicle-associated membrane protein 2; VAMP-2; Synaptobrevin-2; VAMP3; SYB3; Vesicle-associated membrane protein 3; VAMP-3; Cellubrevin; CEB; Synaptobrevin-3; anti-VAMP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of VAMP1/2/3 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Sequence Length
118
Applicable Applications for anti-VAMP1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human VAMP1/2/3. The exact sequence is proprietary.
Preparation and Storage
Shipped and store at 4 degree C for one year. Do not freeze.

Western Blot (WB)

(Western blot analysis of VAMP1/2/3 expression in HepG2 (A), mouse brain (B) whole cell lysates.)

Western Blot (WB) (Western blot analysis of VAMP1/2/3 expression in HepG2 (A), mouse brain (B) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of VAMP1/2/3 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of VAMP1/2/3 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-VAMP1 antibody
Rabbit polyclonal antibody to VAMP1/2/3

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
12,658 Da
NCBI Official Full Name
Vesicle-associated membrane protein 1
NCBI Official Synonym Full Names
vesicle associated membrane protein 1
NCBI Official Symbol
VAMP1
NCBI Official Synonym Symbols
SYB1; SPAX1; VAMP-1
NCBI Protein Information
vesicle-associated membrane protein 1
UniProt Protein Name
Vesicle-associated membrane protein 1
UniProt Gene Name
VAMP1
UniProt Synonym Gene Names
SYB1; VAMP-1
UniProt Entry Name
VAMP1_HUMAN

NCBI Description

Synapotobrevins, syntaxins, and the synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Mutations in this gene are associated with autosomal dominant spastic ataxia 1. Multiple alternative splice variants have been described, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2014]

Uniprot Description

VAMP1: Involved in the targeting and/or fusion of transport vesicles to their target membrane. Belongs to the synaptobrevin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p

Cellular Component: cell junction; cell surface; cytosol; integral to plasma membrane; mitochondrial outer membrane; synaptic vesicle membrane; terminal button

Molecular Function: protein binding; SNAP receptor activity; SNARE binding

Biological Process: vesicle fusion

Disease: Spastic Ataxia 1, Autosomal Dominant

Research Articles on VAMP1

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Product Notes

The VAMP1 vamp1 (Catalog #AAA8241792) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-VAMP1/2/3 Antibody reacts with Human, Mouse, Rat, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's VAMP1/2/3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the VAMP1 vamp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VAMP1/2/3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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