Testing Data
(Lanes: 1:non-transfected cells; 2:V5-tagged empty plasmid; 3:protein A (V5-tagged); 4:protein B (V5-tagged); 5:protein C (V5-tagged); 6:protein D (V5-tagged); Protocol and data courtesy of Dr. Gustavo Gutierrez, Burnham Institute for Medical Research.)
Rabbit V5 Tag Polyclonal Antibody
V5 Tag (AP)
Applications
Western Blot, ELISA
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
V5 Tag; Polyclonal Antibody; V5 Tag (AP); anti-V5 Tag antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes V5 Tag
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-V5 Tag antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
KLH conjugated synthetic peptide encoding the V5 tag (GKPIPNPLLGLDST) was used as antigen.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Testing Data
(Lanes: 1:non-transfected cells; 2:V5-tagged empty plasmid; 3:protein A (V5-tagged); 4:protein B (V5-tagged); 5:protein C (V5-tagged); 6:protein D (V5-tagged); Protocol and data courtesy of Dr. Gustavo Gutierrez, Burnham Institute for Medical Research.)
Testing Data
(Lanes: 1:non-transfected cells; 2:V5-tagged empty plasmid; 3:protein A (V5-tagged); 4:protein B (V5-tagged); 5:protein C (V5-tagged); 6:protein D (V5-tagged); Protocol and data courtesy of Dr. Gustavo Gutierrez, Burnham Institute for Medical Research.)
Western Blot (WB)
(Western Blot analysis of 043700 in 12tag protein (35ug/lane). 12 Tag (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western Blot analysis of 043700 in 12tag protein (35ug/lane). 12 Tag (arrow) was detected using the purified Pab.)
Related Product Information for anti-V5 Tag antibody
V5 is used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. The V5 epitope tag is derived from an epitope present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). Usually the V5 tag is used with all 14aa (GKPIPNPLLGLDST), although it has also been used with a shorter 9aa sequence (IPNPLLGLD).
Product Categories/Family for anti-V5 Tag antibody
References
Dunn C, et al. J Immunol Methods. 1999 Apr 22;224(1-2):141-50.
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Product Notes
The V5 Tag (Catalog #AAA6362169) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's V5 Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the V5 Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "V5 Tag, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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