Rabbit anti-Human USP50 Polyclonal Antibody | anti-USP50 antibody

USP50, ID (USP50, Inactive ubiquitin carboxyl-terminal hydrolase 50, Inactive ubiquitin-specific peptidase 50) (Azide free) (HRP)

Cat. Num. AAA6361997

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: USP50; Polyclonal Antibody; ID (USP50; Inactive ubiquitin carboxyl-terminal hydrolase 50; Inactive ubiquitin-specific peptidase 50) (Azide free) (HRP); Inactive ubiquitin-specific peptidase 50; anti-USP50 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-USP50 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: USP50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-178 amino acids from the Central region of human USP50.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(USP50 Antibody (Center) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the USP50 antibody detected the USP50 protein (arrow).)

Immunohistochemistry (IHC)

(USP50 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of USP50 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-USP50 antibody

Antibodies; Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 45267835
• NCBI GeneID: 373509
• NCBI Accession #: NP_987090.1
• NCBI GenBank Nucleotide #: NM_203494.3
• UniProt Accession #: Q70EL3; E9PP86
• Molecular Weight: 38,383 Da
• NCBI Official Full Name: inactive ubiquitin carboxyl-terminal hydrolase 50
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 50
• NCBI Official Symbol: USP50
• NCBI Protein Information: inactive ubiquitin carboxyl-terminal hydrolase 50
• UniProt Protein Name: Inactive ubiquitin carboxyl-terminal hydrolase 50
• Protein Family: Inactive ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: USP50
• UniProt Entry Name: UBP50_HUMAN

Uniprot Description

USP50: Has no peptidase activity. Belongs to the peptidase C19 family.

Protein type: Ubiquitin-specific protease; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 15q21.1

Biological Process: ubiquitin-dependent protein catabolic process