Rabbit USP4 Polyclonal Antibody | anti-USP4 antibody

USP4 Polyclonal Antibody

Cat. Num. AAA2548032

• Gene Names: USP4; UNP; Unph
• Applications: Western Blot
• Synonyms: USP4; Polyclonal Antibody; USP4 Polyclonal Antibody; MGC149848; MGC149849; UNP; Unph; anti-USP4 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Sequence Length: 313

• Applicable Applications for anti-USP4 antibody: Western Blot (WB)
• Application Notes: WB: 1:500 - 1:2000
• Immunogen: Recombinant protein of human USP4
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Related Product Information for anti-USP4 antibody

Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. USP4 was originally identified during a survey of murine genes near the Mpv20 retroviral insertion site and intially referred to as Ubiquitous Nuclear Protein (UNP). Analysis of the mouse cDNA originally identified Usp4/Unp as a proto-oncogene related to the human tre-2/tre-17/USP6 proto-oncogene (3,4). Usp4/Unp was subsequently observed to contain the conserved Cys and His boxes of the UBP family (5,6) as well as DUB activity (7,8). In a study of primary lung tumor tissue, it was observed that the human homolog of Usp4, USP4/UNPH, had elevated gene expression levels in small cell tumors and adenocarcinomas of the lung, suggesting a causative role for USP4 in neoplasia (6). Another recent study demonstrated overexpression of USP4 in several types of human cancer and that USP4 positively contributes to cell transformation by negatively regulating p53 levels (9). Both murine and human USP4 have been shown to interact with the Rb family of tumor suppressor proteins, providing additional mechanistic evidence of a role for USP4 in cellular transformation (10, 11).

NCBI and Uniprot Product Information

• NCBI GI #: 353523825
• NCBI GeneID: 7375
• NCBI Accession #: NP_001238806.1
• NCBI GenBank Nucleotide #: NM_001251877.1
• UniProt Accession #: Q13107; O43452; O43453; Q08AK8; A8K6Y0; C9IY91
• Molecular Weight: 109kDa
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 4 isoform c
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 4
• NCBI Official Symbol: USP4
• NCBI Official Synonym Symbols: UNP; Unph
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 4
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 4
• UniProt Gene Name: USP4
• UniProt Synonym Gene Names: UNP; UNPH

NCBI Description

The protein encoded by this gene is a protease that deubiquitinates target proteins such as ADORA2A and TRIM21. The encoded protein shuttles between the nucleus and cytoplasm and is involved in maintaining operational fidelity in the endoplasmic reticulum. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

USP4: Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER. Interacts with RB1 (both dephosphorylated and hypophosphorylated forms). Interacts with ADORA2A (via cytoplasmic C-terminus); the interaction is direct. Interacts with RB1, RBL1 and RBL2. Overexpressed in small cell tumors and adenocarcinomas of the lung compared to wild-type lung. Expressed in the hippocampal neurons. Belongs to the peptidase C19 family. USP4 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Oncoprotein; Protease; Ubiquitin conjugating system; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: cytoplasm; cytosol; lysosome; nucleus; plasma membrane

Molecular Function: adenosine receptor binding; identical protein binding; protein binding; ubiquitin-specific protease activity

Biological Process: assembly of spliceosomal tri-snRNP; negative regulation of protein ubiquitination; protein deubiquitination; regulation of protein stability