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Western Blot (WB) (Western Blot (WB) analysis of USP36 polyclonal antibody at 1:500 dilutionLane1:MCF-7 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:3T3-L1 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug))

Rabbit anti-Human, Rat USP36 Polyclonal Antibody | anti-USP36 antibody

USP36 Polyclonal Antibody

Reactivity
Human, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
USP36; Polyclonal Antibody; USP36 Polyclonal Antibody; Ubiquitin carboxyl-terminal hydrolase 36; Deubiquitinating enzyme 36; Ubiquitin thioesterase 36; Ubiquitin-specific-processing protease 36; KIAA1453; anti-USP36 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
USP36 polyclonal antibody detects endogenous levels of USP36 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-USP36 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Synthetic peptide, corresponding to amino acids 500-550 of Human USP36.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of USP36 polyclonal antibody at 1:500 dilutionLane1:MCF-7 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:3T3-L1 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug))

Western Blot (WB) (Western Blot (WB) analysis of USP36 polyclonal antibody at 1:500 dilutionLane1:MCF-7 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:3T3-L1 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug))
Related Product Information for anti-USP36 antibody
Human USP36, encoding a DUB enzyme, was isolated from ovarian cancer cells using RT-PCR and characterized. Identified DUB enzyme activity of USP36 by analyzing its capability to cleave the ubiquitin. Structural and immunoprecipitation analyses revealed for the first time that USP36 contains the PEST motif and is polyubiquitinated.

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
~ 122kDa
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 36
UniProt Gene Name
USP36
UniProt Synonym Gene Names
KIAA1453
UniProt Entry Name
UBP36_HUMAN

Uniprot Description

USP36: May be required for maintaining multiple types of adult stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4). Belongs to the peptidase C19 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.19.12; Nucleolus; Ubiquitin-specific protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleolus; nucleus

Molecular Function: cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination

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Product Notes

The USP36 usp36 (Catalog #AAA3007662) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The USP36 Polyclonal Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's USP36 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the USP36 usp36 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP36, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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