Rabbit anti-Human USP33 Polyclonal Antibody | anti-USP33 antibody

USP33 antibody

Cat. Num. AAA535593

• Gene Names: USP33; VDU1
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: USP33 antibody was purified by affinity chromatography.
• Synonyms: USP33; Polyclonal Antibody; USP33 antibody; Polyclonal USP33; Anti-USP33; USP 33; usp33; VDU1; kiaa1097; USP -33; pVHL-interacting deubiquitinating enzyme 1; anti-USP33 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human
• Purity/Purification: USP33 antibody was purified by affinity chromatography.
• Form/Format: Purified by Immunoaffinity Chromatography and supplied in PBS with 0.1% NaN3.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 942

• Applicable Applications for anti-USP33 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: IHC-P: 5 ug/ml
• Biological Significance: USP 33 is a deubiquitinating enzyme involved in various processes such as cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. It is involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulation of Slit signaling. It also plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2).
• Biohazard Information: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling.
• Immunogen: USP33 antibody was raised in rabbit using a synthetic peptide conjugated to KLH as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -70 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Related Product Information for anti-USP33 antibody

Rabbit polyclonal USP33 antibody

Product Categories/Family for anti-USP33 antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 42516567
• NCBI GeneID: 23032
• NCBI Accession #: NP_055832.3
• NCBI GenBank Nucleotide #: NM_015017.4
• UniProt Accession #: Q8TEY7; Q8TEY6; Q96AV6; Q9H9F0; Q9UPQ5
• Molecular Weight: 93,957 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 33 isoform 1
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 33
• NCBI Official Symbol: USP33
• NCBI Official Synonym Symbols: VDU1
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 33
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 33
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: USP33
• UniProt Synonym Gene Names: KIAA1097; VDU1; hVDU1
• UniProt Entry Name: UBP33_HUMAN

NCBI Description

This gene encodes a deubiquinating enzyme important in a variety of processes, including Slit-dependent cell migration and beta-2 adrenergic receptor signaling. The protein is negatively regulated through ubiquitination by von Hippel-Lindau tumor protein (VHL). Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jun 2012]

Uniprot Description

USP33: Deubiquitinating enzyme involved in various processes such as cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta- arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the peptidase C19 family. USP20/USP33 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Protease; Ubiquitin-specific protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: Golgi apparatus; centrosome; intermediate filament cytoskeleton; perinuclear region of cytoplasm; cytoplasm; VCB complex; autophagic vacuole

Molecular Function: protein binding; ubiquitin binding; G-protein-coupled receptor binding; zinc ion binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; Ral GTPase binding

Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; axon guidance; cell migration; protein deubiquitination; positive regulation of protein binding; endocytosis; regulation of autophagy; cellular response to starvation; negative regulation of protein binding; centrosome duplication; regulation of G-protein coupled receptor protein signaling pathway