Rabbit anti-Human USP33 Polyclonal Antibody | anti-USP33 antibody

USP33, NT (Ubiquitin Carboxyl-terminal Hydrolase 33, Ubiquitin Thiolesterase 33, Ubiquitin-specific Processing Protease 33, Deubiquitinating Enzyme 33, VHL-interacting Deubiquitinating Enzyme 1, hVDU1, KIAA1097, VDU1)

Cat. Num. AAA627719

• Gene Names: USP33; VDU1; KIAA1097; MGC16868
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: USP33; Polyclonal Antibody; NT (Ubiquitin Carboxyl-terminal Hydrolase 33; Ubiquitin Thiolesterase 33; Ubiquitin-specific Processing Protease 33; Deubiquitinating Enzyme 33; VHL-interacting Deubiquitinating Enzyme 1; hVDU1; KIAA1097; VDU1); Anti -USP33; anti-USP33 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human VDU1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-USP33 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human VDU1-II (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-USP33 antibody

Type 2 iodothyronine deiodinase (D2) is an integral membrane selenoenzyme that stimulates the pro-hormone thyroxine (T4) and supplies the majority of the 3,5,3?-triiodothyronine (T3) essential for brain development.1 T4 catalysis accelerates selective conjugation to ubiquitin and thereby renders D2 inactive, a posttranslational feedback mechanism used to maintain acceptable T3 levels.2,3 Ub-D2 was the first recognized substrate for von Hippel-Lindau protein-interacting (pVHL-interacting) deubiquitinating enzyme-1 (VDU1).4 VDU proteins colocalize with D2 in the endoplasmic reticulum, and their coexpression provides D2 resistance to degradation. VDU1 expression is substantially upregulated in brown adipocytes by norepinephrine or cold exposure, further amplifying D2 activity. VDU1 and VDU2 are coexpressed with D2 in many human tissues, including brain, heart, and skeletal muscle, suggesting potential roles in neurological development, cardiac function, and energy management, in addition to thermal homeostasis.VDU1- or VDU2-catalyzed deubiquitination recycles inactive Ub-D2 to its active deubiquitinated form, circumventing the proteasomal degradation pathway. Thus, Ub-D2 can be either reactivated or degraded, with the balance between these two processes influenced by VDU activity.VDU1-catalyzed D2 deubiquitination may be an important participant in the adaptive mechanism that regulates thyroid hormone action. The reversible ubiquitination-dependent mechanism regulating D2 activity permits highly responsive control of thyroid hormone activation.5,6

Product Categories/Family for anti-USP33 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 42516567
• NCBI GeneID: 23032
• NCBI Accession #: NP_055832.3
• NCBI GenBank Nucleotide #: NM_015017.3
• UniProt Accession #: Q8TEY7; Q8TEY6; Q96AV6; Q9H9F0; Q9UPQ5; Q9Y417
• Molecular Weight: 106,727 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 33 isoform 1
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 33
• NCBI Official Symbol: USP33
• NCBI Official Synonym Symbols: VDU1; KIAA1097; MGC16868
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 33; hVDU1; OTTHUMP00000011234; OTTHUMP00000011330; OTTHUMP00000011331; ubiquitin thioesterase 33; deubiquitinating enzyme 33; ubiquitin thiolesterase 33; ubiquitin specific protease 33; VHL-interacting deubiquitinating enzyme 1; ubiquitin-specific-processing protease 33; pVHL-interacting deubiquitinating enzyme 1
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 33
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: USP33
• UniProt Synonym Gene Names: KIAA1097; VDU1
• UniProt Entry Name: UBP33_HUMAN

NCBI Description

This gene encodes a deubiquinating enzyme important in a variety of processes, including Slit-dependent cell migration and beta-2 adrenergic receptor signaling. The protein is negatively regulated through ubiquitination by von Hippel-Lindau tumor protein (VHL). Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jun 2012]

Uniprot Description

USP33: Deubiquitinating enzyme involved in various processes such as cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta- arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the peptidase C19 family. USP20/USP33 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.19.12; Ubiquitin conjugating system; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: Golgi apparatus; centrosome; intermediate filament cytoskeleton; perinuclear region of cytoplasm; cytoplasm; VCB complex; autophagic vacuole

Molecular Function: protein binding; ubiquitin binding; G-protein-coupled receptor binding; zinc ion binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; Ral GTPase binding

Biological Process: ubiquitin-dependent protein catabolic process; axon guidance; proteasomal ubiquitin-dependent protein catabolic process; cell migration; protein deubiquitination; positive regulation of protein binding; regulation of autophagy; endocytosis; negative regulation of protein binding; cellular response to starvation; centrosome duplication; regulation of G-protein coupled receptor protein signaling pathway