Rabbit anti-Human USP28 Polyclonal Antibody | anti-USP28 antibody

USP28, CT (Ubiquitin Carboxyl-terminal Hydrolase 28, Ubiquitin Thiolesterase 28, Ubiquitin-specific Processing Protease 28, Deubiquitinating Enzyme 28, KIAA1515)

Cat. Num. AAA627727

• Gene Names: USP28; KIAA1515
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: USP28; Polyclonal Antibody; CT (Ubiquitin Carboxyl-terminal Hydrolase 28; Ubiquitin Thiolesterase 28; Ubiquitin-specific Processing Protease 28; Deubiquitinating Enzyme 28; KIAA1515); Anti -USP28; anti-USP28 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human USP28.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-USP28 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human USP28 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-USP28 antibody

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Product Categories/Family for anti-USP28 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 16507200
• NCBI GeneID: 57646
• NCBI Accession #: NP_065937.1
• NCBI GenBank Nucleotide #: NM_020886.2
• UniProt Accession #: Q96RU2; Q9P213; B0YJC0; B0YJC1; Q6NZX9; Q96SV4
• Molecular Weight: 122,491 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 28
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 28
• NCBI Official Symbol: USP28
• NCBI Official Synonym Symbols: KIAA1515
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 28; OTTHUMP00000238295; OTTHUMP00000238297; ubiquitin thioesterase 28; deubiquitinating enzyme 28; ubiquitin thiolesterase 28; ubiquitin specific protease 28; ubiquitin-specific-processing protease 28
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 28
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: USP28
• UniProt Synonym Gene Names: KIAA1515
• UniProt Entry Name: UBP28_HUMAN

NCBI Description

The ubiquitin-dependent protein degradation pathway is essential for proteolysis of intracellular proteins and peptides. Enzymes that remove ubiquitin from ubiquitin-conjugated peptides, like USP28, affect the fate and degradation of intracellular proteins and are essential for maintenance of cell-free ubiquitin pools (Valero et al., 2001).[supplied by OMIM]

Uniprot Description

USP28: a peptidase of the C19 family. USP28 and FBXW7 regulate Myc protein stability in response to DNA damage. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-specific protease; EC 3.4.19.12; Protease

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: nucleoplasm; protein complex; nucleolus; nucleus

Molecular Function: protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; cell proliferation; protein deubiquitination; DNA damage checkpoint; response to ionizing radiation; DNA repair; response to DNA damage stimulus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis