Rabbit anti-Human USP2 Polyclonal Antibody | anti-USP2 antibody

USP2, CT (L523) (Ubiquitin Carboxyl-terminal Hydrolase 2, Ubiquitin Thiolesterase 2, Ubiquitin-specific Processing Protease 2, Deubiquitinating Enzyme 2, 41kD Ubiquitin-specific Protease, UBP41) (APC)

Cat. Num. AAA6504970

• Gene Names: USP2; USP9; UBP41
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: USP2; Polyclonal Antibody; CT (L523) (Ubiquitin Carboxyl-terminal Hydrolase 2; Ubiquitin Thiolesterase 2; Ubiquitin-specific Processing Protease 2; Deubiquitinating Enzyme 2; 41kD Ubiquitin-specific Protease; UBP41) (APC); anti-USP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human USP2.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-USP2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human USP2 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-USP2 Antibody (C-term L523) in K562 cell line lysates (35ug/lane).USP2(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with USP2 antibody (C-term L523), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of USP2 (arrow) using rabbit polyclonal USP2 Antibody (C-term L523). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the USP2 gene.)

Product Categories/Family for anti-USP2 antibody

Antibodies; Ubiquitin

References

1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 188528692
• NCBI GeneID: 9099
• NCBI Accession #: NP_004196
• NCBI GenBank Nucleotide #: NM_004205.4
• UniProt Accession #: O75604; Q8IUM2; Q8IW04; Q96MB9; Q9BQ21; B0YJB8; E9PPM2
• Molecular Weight: 45,241 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 2 isoform a
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 2
• NCBI Official Symbol: USP2
• NCBI Official Synonym Symbols: USP9; UBP41
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 2
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 2
• Protein Family: Protein
• UniProt Gene Name: USP2
• UniProt Synonym Gene Names: UBP41
• UniProt Entry Name: UBP2_HUMAN

NCBI Description

This gene encodes a member of the family of de-ubiquitinating enzymes, which belongs to the peptidase C19 superfamily. The encoded protein is a ubiquitin-specific protease which is required for TNF-alpha (tumor necrosis factor alpha) -induced NF-kB (nuclear factor kB) signaling. This protein deubiquitinates polyubiquitinated target proteins such as fatty acid synthase, murine double minute 2 (MDM2), MDM4/MDMX and cyclin D1. MDM2 and MDM4 are negative regulators of the p53 tumor suppressor and cyclin D1 is required for cell cycle G1/S transition. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

USP2: Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Isoform 1 and isoform 4 possess both ubiquitin-specific peptidase and isopeptidase activities. Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity. Has no deubiquitinase activity against p53/TP53. Prevents MDM2-mediated degradation of MDM4. Plays a role in the G1/S cell-cycle progression in normal and cancer cells. Plays a role in the regulation of myogenic differentiation of embryonic muscle cells. Homooligomer. Found in trimeric complex with MDM2 and MDM4 and UPB2. Interacts with CCND1; the interaction is direct and promotes its stabilization by antagonizing ubiquitin-dependent degradation. Interacts (via N-terminus and C- terminus) with MDM2. Interacts with MDM4. Down-regulated by cisplatin. Expressed in mesangial cells of the kidney and in different types of glomerulonephritides. Cleavage is inhibited by ubiquitin in a dosage- dependent manner. Cleavage is blocked by ubiquitin aldehyde. Belongs to the peptidase C19 family. USP2 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-specific protease; Protease; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: centrosome; perinuclear region of cytoplasm; cytoplasm; cell cortex; nucleus

Molecular Function: identical protein binding; protein binding; cyclin binding; ubiquitin protein ligase binding; metal ion binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; negative regulation of skeletal muscle development; muscle development; protein deubiquitination; protein stabilization; positive regulation of skeletal muscle development; negative regulation of transcription from RNA polymerase II promoter; positive regulation of mitotic cell cycle; cell cycle; circadian behavior; entrainment of circadian clock by photoperiod; locomotor rhythm; circadian regulation of gene expression