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Western Blot (WB) (Western blot analysis USP17L using HuvEc whole cell lysates)

Rabbit USP17L Polyclonal Antibody | anti-USP17L antibody

USP17L Antibody

Gene Names
USP17L1; USP17L1P
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Peptide affinity purification
Synonyms
USP17L; Polyclonal Antibody; USP17L Antibody; anti-USP17L antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
USP17L antibody detects endogenous levels of total USP17L
Purity/Purification
Peptide affinity purification
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
~1mg/mL (varies by lot)
Sequence Length
530
Applicable Applications for anti-USP17L antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IF/ICC: 1:100-1:500
ELISA (peptide): 1:20000-1:40000
The optimal dilution should be determined by the end user.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human USP17L, corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis USP17L using HuvEc whole cell lysates)

Western Blot (WB) (Western blot analysis USP17L using HuvEc whole cell lysates)

Western Blot (WB)

(Western blot analysis of extracts from mouse brain, using USP17L Antibody.)

Western Blot (WB) (Western blot analysis of extracts from mouse brain, using USP17L Antibody.)

Immunofluorescence (IF)

(MBS9610298 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9610298) and mouse anti-beta tubulin Ab(MBS9610328) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).)

Immunofluorescence (IF) (MBS9610298 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9610298) and mouse anti-beta tubulin Ab(MBS9610328) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).)
Related Product Information for anti-USP17L antibody
Function: Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes that may include cell proliferation, progression through the cell cycle, apoptosis, cell migration, and the cellular response to viral infection.
Similarity: Belongs to the peptidase C19 family. USP17 subfamily.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
60 kDa
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 17-like protein 1
NCBI Official Synonym Full Names
ubiquitin specific peptidase 17-like family member 1
NCBI Official Symbol
USP17L1
NCBI Official Synonym Symbols
USP17L1P
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 17-like protein 1
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 17-like protein 1
UniProt Gene Name
USP17L1
UniProt Synonym Gene Names
USP17L; USP17L1P

Uniprot Description

Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes that may include cell proliferation, progression through the cell cycle, apoptosis, cell migration, and the cellular response to viral infection.

Similar Products

Product Notes

The USP17L usp17l1 (Catalog #AAA9610298) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The USP17L Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's USP17L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000 IF/ICC: 1:100-1:500 ELISA (peptide): 1:20000-1:40000 The optimal dilution should be determined by the end user. IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. . Researchers should empirically determine the suitability of the USP17L usp17l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP17L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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