Western Blot (WB)
(Western Blot analysis of PON1 (arrow) in 293 cell lysates (2ug/lane) using MBS649722 either nontransfected (Lane 1) or transiently transfected with the PON1 gene (Lane 2) .)
Rabbit anti-Human PON1 Polyclonal Antibody | anti-PON1 antibody
PON1, ID (PON1, PON, Serum paraoxonase/arylesterase 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Immunohistochemistry: 1:10-50
Western Blot (WB)
(Western Blot analysis of PON1 (arrow) in 293 cell lysates (2ug/lane) using MBS649722 either nontransfected (Lane 1) or transiently transfected with the PON1 gene (Lane 2) .)
Western Blot (WB)
(Western Blot analysis of PON1 (arrow) in 293 cell lysates (2ug/lane) using MBS649722 either nontransfected (Lane 1) or transiently transfected with the PON1 gene (Lane 2) .)
Western Blot (WB)
(Western Blot analysis of lysate from human blood plasma tissue lysate (35ug), MBS649722 (1:1000). A goat anti-rabbit IgG H&L (HRP) at 1:5000 was used as the secondary antibody.)
Western Blot (WB)
(Western Blot analysis of lysate from human blood plasma tissue lysate (35ug), MBS649722 (1:1000). A goat anti-rabbit IgG H&L (HRP) at 1:5000 was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue using MBS649722 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS649722 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue using MBS649722 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS649722 for immunohistochemistry.)
NCBI and Uniprot Product Information
NCBI Description
The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq, Oct 2008]
Uniprot Description
Function: Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. Ref.18 Ref.20
Catalytic activity: A phenyl acetate + H2O = a phenol + acetate. Ref.3 Ref.15 Ref.16 Ref.20 Ref.24An aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol. Ref.3 Ref.15 Ref.16 Ref.20 Ref.24An N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine. Ref.3 Ref.15 Ref.16 Ref.20 Ref.24
Cofactor: Binds 2 calcium ions per subunit.
Subunit structure: Homodimer. Heterooligomer with phosphate-binding protein (HPBP). Interacts with CLU. Ref.14 Ref.20 Ref.22
Subcellular location: Secreted › extracellular space.
Tissue specificity: Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas. Ref.13 Ref.14
Post-translational modification: Glycosylated.The signal sequence is not cleaved.Present in two forms, form B contains a disulfide bond, form A does not.
Polymorphism: The allelic form of the enzyme with Gln-192 (allozyme A) hydrolyzes paraoxon with a low turnover number and the one with Arg-192 (allozyme B) with a high turnover number.
Involvement in disease: Microvascular complications of diabetes 5 (MVCD5) [MIM:612633]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Homozygosity for the Leu-55 allele is strongly associated with the development of retinal disease in diabetic patients.
Miscellaneous: The preferential association of PON1 with HDL is mediated in part by its signal peptide, by binding phospholipids directly, rather than binding apo AI. The retained signal peptide may allow transfer of the protein between phospholipid surfaces.
Sequence similarities: Belongs to the paraoxonase family.
Research Articles on PON1
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Product Notes
The PON1 pon1 (Catalog #AAA649722) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PON1, ID (PON1, PON, Serum paraoxonase/arylesterase 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PON1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Flow Cytometry: 1:10-50 Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the PON1 pon1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PON1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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