Rabbit Usp14 Polyclonal Antibody | anti-Usp14 antibody

Usp14 Polyclonal Antibody

Cat. Num. AAA3013649

• Gene Names: USP14; TGT
• Reactivity: Human, Zebrafish
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: Usp14; Polyclonal Antibody; Usp14 Polyclonal Antibody; USP14; TGT; Deubiquitinating enzyme 14; tRNA guanine transglycosylase 60 kD subunit; tRNA guanine transglycosylase; Ubiquitin carboxyl terminal hydrolase 14; Ubiquitin carboxyl-terminal hydrolase 14; Ubiquitin specific peptidase 14; Ubiquitin specific processing protease 14; Ubiquitin specific protease 14; Ubiquitin thiolesterase 14; Ubiquitin-specific-processing protease 14; UBP14; USP 14; anti-Usp14 antibody

• Host: Rabbit
• Reactivity: Human, Zebrafish
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Usp14 polyclonal antibody detects endogenous levels of Usp14 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 349

• Applicable Applications for anti-Usp14 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:2,000; ICC: 1:50-1:200; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: Recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of USP14 on K562 (1) and Hela (2) cell lysate using anti-USP14 antibody at 1/1,000 dilution.)

Immunocytochemistry (ICC)

(ICC staining USP14 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-Usp14 antibody

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP14 (ubiquitin specific peptidase 14), also known as TGT (tRNA-guanine transglycosylase), is a cytoplasmic protein that belongs to the ubiquitin-specific processing family of deubiquitinating enzymes. Existing as a homodimer within the cell, USP14 functions to cleave ubiquitin residues from both ubiquitinylated proteins and ubiquitin-fused precursors, thereby saving these proteins from proteasomal degradation. In mice, defects or mutations in the gene encoding USP14 cause retarded growth or fetal death, indicating that USP14 plays a key role in early developmental processes. Multiple isoforms of USP14 are expressed due to alternative splicing events.

NCBI and Uniprot Product Information

• NCBI GI #: 119622143
• NCBI GeneID: 9097
• UniProt Accession #: P54578
• Molecular Weight: 56kDa
• NCBI Official Full Name: ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase), isoform CRA_c
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 14
• NCBI Official Symbol: USP14
• NCBI Official Synonym Symbols: TGT
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 14
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 14
• UniProt Gene Name: USP14
• UniProt Synonym Gene Names: TGT
• UniProt Entry Name: UBP14_HUMAN

NCBI Description

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

USP14: Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). Homodimer (Potential). Associates with the 26S proteasome. Interacts with FANCC, CXCR4 and ERN1. Belongs to the peptidase C19 family. USP14/UBP6 subfamily.

Protein type: Protease; Ubiquitin conjugating system; Ubiquitin-specific protease; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: proteasome complex; cell surface; cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane; synapse; nucleus

Molecular Function: protein binding; tRNA guanylyltransferase activity; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; endopeptidase inhibitor activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; synaptic transmission; protein deubiquitination; regulation of chemotaxis