Western Blot (WB)
(The anti-USP12 Pab is used in Western blot to detect USP12 in mouse kidney tissue lysate.)
Rabbit anti-Human, Mouse USP12 Polyclonal Antibody | anti-USP12 antibody
USP12, NT (Ubiquitin Carboxyl-terminal Hydrolase 12, Ubiquitin Thiolesterase 12, Ubiquitin-specific Processing Protease 12, Deubiquitinating Enzyme 12, Ubiquitin Hydrolyzing Enzyme 1, UBH1, USP12L1) (Azide free) (HRP)
Reactivity
Human, Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
USP12; Polyclonal Antibody; NT (Ubiquitin Carboxyl-terminal Hydrolase 12; Ubiquitin Thiolesterase 12; Ubiquitin-specific Processing Protease 12; Deubiquitinating Enzyme 12; Ubiquitin Hydrolyzing Enzyme 1; UBH1; USP12L1) (Azide free) (HRP); anti-USP12 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human USP12. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-USP12 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-1:100
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human USP12 (KLH).
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(The anti-USP12 Pab is used in Western blot to detect USP12 in mouse kidney tissue lysate.)
Western Blot (WB)
(The anti-USP12 Pab is used in Western blot to detect USP12 in mouse kidney tissue lysate.)
Western Blot (WB)
(Western blot analysis of USP12 Antibody (N-term) in CEM cell line lysates (35ug/lane). USP12 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of USP12 Antibody (N-term) in CEM cell line lysates (35ug/lane). USP12 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Product Categories/Family for anti-USP12 antibody
References
1. Hansen-Hagge, T.E., et al., Genomics 49(3):411-418 (1998).
Similar Products
USP12 Antibody
Host: Rabbit
Reactivity: Human, Mouse
Apps: Immunohistochemistry, ELISA, Western Blot
USP12 Antibody
Host: Rabbit
Reactivity: Human, Mouse
Apps: Immunohistochemistry, ELISA, Western Blot
Product Notes
The USP12 (Catalog #AAA6505127) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The USP12, NT (Ubiquitin Carboxyl-terminal Hydrolase 12, Ubiquitin Thiolesterase 12, Ubiquitin-specific Processing Protease 12, Deubiquitinating Enzyme 12, Ubiquitin Hydrolyzing Enzyme 1, UBH1, USP12L1) (Azide free) (HRP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's USP12 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-1:100 ELISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the USP12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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