Rabbit anti-Human USP1 Polyclonal Antibody | anti-USP1 antibody

USP1, CT (Ubiquitin Carboxyl-terminal Hydrolase 1, Ubiquitin Thioesterase 1, Ubiquitin-specific-processing Protease 1, Deubiquitinating Enzyme 1, hUBP) (Azide free) (HRP)

Cat. Num. AAA6504940

• Gene Names: USP1; UBP
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: USP1; Polyclonal Antibody; CT (Ubiquitin Carboxyl-terminal Hydrolase 1; Ubiquitin Thioesterase 1; Ubiquitin-specific-processing Protease 1; Deubiquitinating Enzyme 1; hUBP) (Azide free) (HRP); anti-USP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human USP1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-USP1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human USP1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-USP1 Pab in HepG2 cell line lysate (35ug/lane). USP1(arrow) was detected using the purified Pab)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-USP1 antibody

Antibodies; Ubiquitin

References

1. Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003). 2. Fujiwara, T., et al., Genomics 54(1):155-158 (1998). 3. D'Andrea, A., et al., Crit. Rev. Biochem. Mol. Biol. 33(5):337-352 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 31543910
• NCBI GeneID: 7398
• NCBI Accession #: NP_003359
• NCBI GenBank Nucleotide #: NM_003368
• UniProt Accession #: O94782
• Molecular Weight: 88kDa
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 1
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 1
• NCBI Official Symbol: USP1
• NCBI Official Synonym Symbols: UBP
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 1
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 1
• Protein Family: UDP-sugar pyrophosphorylase
• UniProt Gene Name: USP1
• UniProt Synonym Gene Names: hUBP
• UniProt Entry Name: UBP1_HUMAN

NCBI Description

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. The protein specifically deubiquitinates a protein in the Fanconi anemia (FA) DNA repair pathway. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

USP1: Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Interacts with FANCD2 and PCNA. Interacts with WDR48. Down-regulated following DNA damage. Belongs to the peptidase C19 family.

Protein type: EC 3.4.19.12; Protease; Ubiquitin conjugating system; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: regulation of DNA repair; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; DNA repair; skeletal development; response to UV