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Western Blot (WB) (Western blot analysis of USF2 expression in HeLa (A), Jurkat (B), K562 (C), A431 (D), NIH3T3 (E) whole cell lysates.)

Rabbit USF2 Polyclonal Antibody | anti-USF2 antibody

Anti-USF2 Antibody

Gene Names
USF2; FIP; bHLHb12
Reactivity
Human, Bovine, Pig
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
USF2; Polyclonal Antibody; Anti-USF2 Antibody; BHLHB12; Upstream stimulatory factor 2; Class B basic helix-loop-helix protein 12; bHLHb12; FOS-interacting protein; FIP; Major late transcription factor 2; Upstream transcription factor 2; anti-USF2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Bovine, Pig
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of USF2 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
346
Applicable Applications for anti-USF2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human USF2. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of USF2 expression in HeLa (A), Jurkat (B), K562 (C), A431 (D), NIH3T3 (E) whole cell lysates.)

Western Blot (WB) (Western blot analysis of USF2 expression in HeLa (A), Jurkat (B), K562 (C), A431 (D), NIH3T3 (E) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of USF2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of USF2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-USF2 antibody
Rabbit polyclonal antibody to USF2

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23,988 Da
NCBI Official Full Name
upstream stimulatory factor 2 isoform 1
NCBI Official Synonym Full Names
upstream transcription factor 2, c-fos interacting
NCBI Official Symbol
USF2
NCBI Official Synonym Symbols
FIP; bHLHb12
NCBI Protein Information
upstream stimulatory factor 2; FOS-interacting protein; c-fos interacting protein; major late transcription factor 2; class B basic helix-loop-helix protein 12
UniProt Protein Name
Upstream stimulatory factor 2
UniProt Gene Name
USF2
UniProt Synonym Gene Names
BHLHB12; bHLHb12; FIP
UniProt Entry Name
USF2_HUMAN

NCBI Description

This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

USF2: Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: nucleoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; protein heterodimerization activity; sequence-specific DNA binding; double-stranded DNA binding; bHLH transcription factor binding; transcription factor activity

Biological Process: lactation; transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter by glucose; late viral mRNA transcription; lipid homeostasis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter by glucose

Research Articles on USF2

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Product Notes

The USF2 usf2 (Catalog #AAA821719) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-USF2 Antibody reacts with Human, Bovine, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's USF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000), IH (1/100 - 1/200). Researchers should empirically determine the suitability of the USF2 usf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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