Western Blot (WB)
(Western blot analysis URM1 using LOVO whole cell lysates)
Rabbit URM1 Polyclonal Antibody | anti-URM1 antibody
URM1 Antibody
Gene Names
URM1; C9orf74
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
URM1; Polyclonal Antibody; URM1 Antibody; 2900073H19Rik; C9orf74; Chromosome 9 open reading frame 74; MGC2668; RGD1306599; RP11 339B21.4; RP23-443G7.1; Ubiquitin related modifier 1; Ubiquitin related modifier 1 homolog; Ubiquitin-related modifier 1 homolog; Urm 1; URM1_HUMAN; anti-URM1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
URM1 antibody detects endogenous levels of total URM1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
146
Applicable Applications for anti-URM1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human URM1
Subcellular Location
Cytoplasm.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (100%), Bovine (92%), Horse (100%), Sheep (92%), Rabbit (92%), Dog (92%), Chicken (100%), Xenopus (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis URM1 using LOVO whole cell lysates)
Western Blot (WB)
(Western blot analysis URM1 using LOVO whole cell lysates)
Related Product Information for anti-URM1 antibody
Function: Acts as a sulfur carrier required for 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm5S2U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.
Subunit Structure: Component of a complex at least composed of URM1, CTU2/NCS2 and CTU1/ATPBD3.
Post-translational Modifications: C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3.
Similarity: Belongs to the URM1 family.
Subunit Structure: Component of a complex at least composed of URM1, CTU2/NCS2 and CTU1/ATPBD3.
Post-translational Modifications: C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3.
Similarity: Belongs to the URM1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 11 kDa
Predicted: 12 kDa
Predicted: 12 kDa
NCBI Official Full Name
ubiquitin-related modifier 1 isoform b
NCBI Official Synonym Full Names
ubiquitin related modifier 1
NCBI Official Symbol
URM1
NCBI Official Synonym Symbols
C9orf74
NCBI Protein Information
ubiquitin-related modifier 1
UniProt Protein Name
Ubiquitin-related modifier 1
Protein Family
UniProt Gene Name
URM1
UniProt Synonym Gene Names
C9orf74
Uniprot Description
Acts as a sulfur carrier required for 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm5S2U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.
Research Articles on URM1
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Product Notes
The URM1 urm1 (Catalog #AAA9608631) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The URM1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's URM1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the URM1 urm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "URM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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