Rabbit anti-Human Uridine Monophosphate Kinase 2 Polyclonal Antibody | anti-UCK2 antibody

Uridine Monophosphate Kinase 2, CT (Cytidine monophosphokinase 2, Testis-specific protein, UCK 2, UK, UMPK, Uridine-cytidine kinase 2, Uridine monophosphokinase 2) (Azide free) (HRP)

Cat. Num. AAA6504874

• Gene Names: UCK2; UK; UMPK; TSA903
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Uridine Monophosphate Kinase 2; Polyclonal Antibody; CT (Cytidine monophosphokinase 2; Testis-specific protein; UCK 2; UK; UMPK; Uridine-cytidine kinase 2; Uridine monophosphokinase 2) (Azide free) (HRP); anti-UCK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UCK2.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-UCK2 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human UCK2 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of UCK2 (arrow) using rabbit polyclonal hUCK2-C241. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UCK2 gene.)

Immunohistochemistry (IHC)

(UCK2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UCK2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(UCK2 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-UCK2 antibody

Antibodies; Protein Kinases

References

1. Murata, D., et al., Drug Metab. Dispos. 32(10):1178-1182 (2004). 2. Pasti, C., et al., Eur. J. Biochem. 270(8):1784-1790 (2003). 3. Liou, J.Y., et al., Cancer Res. 62(6):1624-1631 (2002). 4. Pearman, A.T., et al., Life Sci. 69(20):2361-2370 (2001). 5. Van Rompay, A.R., et al., Mol. Pharmacol. 56(3):562-569 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 18699734
• NCBI GeneID: 7371
• NCBI Accession #: NP_036606
• NCBI GenBank Nucleotide #: NM_012474.4
• UniProt Accession #: Q9BZX2; Q5VV91; Q7KZV3; Q92528; Q96KG5; Q9BU42
• Molecular Weight: 30.3kDa (269aa), confirmed by MALDI-TOF
• NCBI Official Full Name: uridine-cytidine kinase 2
• NCBI Official Synonym Full Names: uridine-cytidine kinase 2
• NCBI Official Symbol: UCK2
• NCBI Official Synonym Symbols: UK; UMPK; TSA903
• NCBI Protein Information: uridine-cytidine kinase 2
• UniProt Protein Name: Uridine-cytidine kinase 2
• Protein Family: Uridine-cytidine kinase
• UniProt Gene Name: UCK2
• UniProt Synonym Gene Names: UMPK; UCK 2
• UniProt Entry Name: UCK2_HUMAN

NCBI Description

This gene encodes a pyrimidine ribonucleoside kinase. The encoded protein (EC 2.7.1.48) catalyzes phosphorylation of uridine and cytidine to uridine monophosphate (UMP) and cytidine monophosphate (CMP), respectively.[provided by RefSeq, Oct 2010]

Uniprot Description

UCK2: Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor. Can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4- thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)- benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5- methylcytidine, and N(4)-anisoylcytidine. Belongs to the uridine kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.48; Nucleotide Metabolism - pyrimidine; Kinase, other; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: intracellular membrane-bound organelle; cytosol

Molecular Function: nucleoside kinase activity; uridine kinase activity; ATP binding

Biological Process: pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; CMP salvage; pyrimidine nucleoside salvage; feeding behavior; response to axon injury; phosphorylation