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Western Blot (WB) (Western blot analysis of lysates from SK-BR-3, HepG2 cell line (from left to right), using RAB32 Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

Rabbit anti-Human RAB32 Polyclonal Antibody | anti-RAB32 antibody

RAB32 Antibody (N-term)

Reactivity
Human
Applications
Western Blot, ELISA
Synonyms
RAB32; Polyclonal Antibody; RAB32 Antibody (N-term); Ras-related protein Rab-32; anti-RAB32 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This RAB32 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-28 amino acids from the N-terminal region of human RAB32.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
225
Applicable Applications for anti-RAB32 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000
Antigen Source
Human
Antigen Region
2-28
Antigen Type
Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysates from SK-BR-3, HepG2 cell line (from left to right), using RAB32 Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

Western Blot (WB) (Western blot analysis of lysates from SK-BR-3, HepG2 cell line (from left to right), using RAB32 Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Related Product Information for anti-RAB32 antibody
Description: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Background: Small GTP-binding proteins of the RAB family, such as RAB32, play essential roles in vesicle and granule targeting (Bao et al., 2002 [PubMed 11784320]).
References
Hirota, Y., et al. Cell. Mol. Life Sci. 66(17):2913-2932(2009). Shibata, D., et al. Int. J. Cancer 119(4):801-806(2006). Mungall, A.J., et al. Nature 425(6960):805-811(2003). Alto, N.M., et al. J. Cell Biol. 158(4):659-668(2002). Bao, X., et al. Eur. J. Biochem. 269(1):259-271(2002).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24997
NCBI Official Full Name
ras-related protein Rab-32
NCBI Official Synonym Full Names
RAB32, member RAS oncogene family
NCBI Official Symbol
RAB32
NCBI Protein Information
ras-related protein Rab-32
UniProt Protein Name
Ras-related protein Rab-32
Protein Family
UniProt Gene Name
RAB32
UniProt Entry Name
RAB32_HUMAN

NCBI Description

The protein encoded by this gene anchors the type II regulatory subunit of protein kinase A to the mitochondrion and aids in mitochondrial fission. The encoded protein also appears to be involved in autophagy and melanosome secretion. Variations in this gene may be linked to leprosy. [provided by RefSeq, Dec 2015]

Uniprot Description

RAB32: Acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A and anchoring it to the mitochondrion. Also involved in synchronization of mitochondrial fission. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric; G protein; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 6q24.3

Cellular Component: phagocytic vesicle membrane; mitochondrion; membrane; early endosome; melanosome; phagocytic vesicle; trans-Golgi network

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; GTP-dependent protein binding

Biological Process: intracellular protein transport; antigen processing and presentation; metabolic process; Rab protein signal transduction

Research Articles on RAB32

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Product Notes

The RAB32 rab32 (Catalog #AAA9231620) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAB32 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAB32 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000. Researchers should empirically determine the suitability of the RAB32 rab32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB32, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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