Rabbit anti-Human, Mouse Hypoxia Inducible Factor 1 alpha Polyclonal Antibody | anti-HIF1A antibody

Hypoxia Inducible Factor 1 alpha, CT (Hypoxia-inducible Factor 1-alpha, HIF1-alpha, HIF-1alpha, HIF-1-alpha, HIF1A, HIF1, ARNT-interacting Protein, Basic-helix-loop-helix-PAS Protein MOP1, Class E Basic Helix-loop-helix Protein 78, bHLHe78, Member of PAS

Cat. Num. AAA6481321

• Gene Names: HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
• Reactivity: Human, Mouse
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Hypoxia Inducible Factor 1 alpha; Polyclonal Antibody; CT (Hypoxia-inducible Factor 1-alpha; HIF1-alpha; HIF-1alpha; HIF-1-alpha; HIF1A; HIF1; ARNT-interacting Protein; Basic-helix-loop-helix-PAS Protein MOP1; Class E Basic Helix-loop-helix Protein 78; bHLHe78; Member of PAS; Hypoxia Inducible Factor 1; alpha Subunit (Basic Helix-loop-helix Transcription Factor); anti-HIF1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HIF1A at ~92kD. Species Crossreactivity: mouse
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-HIF1A antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: IF: 1:10-1:50; WB: 1:100-1:500; IHC-P: 1:10-1:50; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa736-765 in the C-terminal region of human HIF1A, UniProt Accession #NP_001521; Q16665 Cellular Localization: Cytoplasm, Nucleus
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HIF1Alpha Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). HIF1Alpha (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of HIF1Alpha Antibody (C-term) in mouse cerebellum tissue lysates (35ug/lane). HIF1Alpha (arrow) was detected using the purified Pab.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HIF1Alpha Antibody (C-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIF1Alpha antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohis)

Product Categories/Family for anti-HIF1A antibody

Antibodies; Transcription Factors

References

1. Favaro, E., Am. J. Pathol. 173(4), 1186-1201 (2008). 2. Alexandru, G., Cell 134(5), 804-816 (2008). 3. Linke, S., J. Biol. Chem. 279(14), 14391-14397 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4504385
• NCBI GeneID: 3091
• NCBI Accession #: NP_001521
• NCBI GenBank Nucleotide #: NM_001530.3
• UniProt Accession #: Q16665; Q53XP6; Q96PT9; Q9UPB1; C0LZJ3
• Molecular Weight: 32.8kDa (298aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: hypoxia-inducible factor 1-alpha isoform 1
• NCBI Official Synonym Full Names: hypoxia inducible factor 1 alpha subunit
• NCBI Official Symbol: HIF1A
• NCBI Official Synonym Symbols: HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
• NCBI Protein Information: hypoxia-inducible factor 1-alpha
• UniProt Protein Name: Hypoxia-inducible factor 1-alpha
• Protein Family: Hypoxia-inducible factor
• UniProt Gene Name: HIF1A
• UniProt Synonym Gene Names: BHLHE78; MOP1; PASD8; HIF-1-alpha; HIF1-alpha; bHLHe78
• UniProt Entry Name: HIF1A_HUMAN

NCBI Description

This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

HIF1A: a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. Interacts with the HIF1A beta/ARNT subunit; heterodimerization is required for DNA binding. Interacts with COPS5; the interaction increases the transcriptional activity of HIF1A through increased stability. Interacts with EP300 (via TAZ-type 1 domains); the interaction is stimulated in response to hypoxia and inhibited by CITED2. Interacts with CREBBP (via TAZ-type 1 domains). Interacts with NCOA1, NCOA2, APEX and HSP90. Interacts (hydroxylated within the ODD domain) with VHLL (via beta domain); the interaction, leads to polyubiquitination and subsequent HIF1A proteasomal degradation. During hypoxia, sumoylated HIF1A also binds VHL; the interaction promotes the ubiquitination of HIF1A. Interacts with SENP1; the interaction desumoylates HIF1A resulting in stabilization and activation of transcription. Interacts (Via the ODD domain) with ARD1A; the interaction appears not to acetylate HIF1A nor have any affect on protein stability, during hypoxia. Interacts with RWDD3; the interaction enhances HIF1A sumoylation. Interacts with TSGA10. Interacts with RORA (via the DNA binding domain); the interaction enhances HIF1A transcription under hypoxia through increasing protein stability. Interaction with PSMA7 inhibits the transactivation activity of HIF1A under both normoxic and hypoxia- mimicking conditions. Interacts with USP20. Interacts with RACK1; promotes HIF1A ubiquitination and proteasome- mediated degradation. Interacts (via N-terminus) with USP19. Under reduced oxygen tension. Induced also by various receptor-mediated factors such as growth factors, cytokines, and circulatory factors such as PDGF, EGF, FGF2, IGF2, TGFB1, HGF, TNF, IL1B, angiotensin-2 and thrombin. However, this induction is less intense than that stimulated by hypoxia. Repressed by HIPK2 and LIMD1. Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding; Autophagy

Chromosomal Location of Human Ortholog: 14q23.2

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleolus; nuclear speck; nucleus; cytosol

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; histone deacetylase binding; Hsp90 protein binding; protein kinase binding; transcription factor binding; histone acetyltransferase binding; signal transducer activity; protein binding; enzyme binding; protein heterodimerization activity; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription factor activity; nuclear hormone receptor binding

Biological Process: lactation; oxygen homeostasis; embryonic placenta development; response to muscle activity; cellular iron ion homeostasis; positive regulation of transcription, DNA-dependent; signal transduction; glucose homeostasis; positive regulation of vascular endothelial growth factor receptor signaling pathway; muscle maintenance; negative regulation of bone mineralization; connective tissue replacement during inflammatory response; elastin metabolic process; axon transport of mitochondrion; regulation of transcription, DNA-dependent; visual learning; heart looping; angiogenesis; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; neural crest cell migration; negative regulation of growth; hemoglobin biosynthetic process; positive regulation of neuroblast proliferation; regulation of transforming growth factor-beta2 production; Notch signaling pathway; negative regulation of TOR signaling pathway; collagen metabolic process; embryonic hemopoiesis; positive regulation of nitric-oxide synthase activity; positive regulation of erythrocyte differentiation; B-1 B cell homeostasis; digestive tract morphogenesis; mRNA transcription from RNA polymerase II promoter; positive regulation of chemokine production; positive regulation of angiogenesis; neural fold elevation formation; regulation of gene expression; cartilage development; positive regulation of hormone biosynthetic process; positive regulation of glycolysis; lactate metabolic process; response to hypoxia; epithelial to mesenchymal transition; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development