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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using UPF1 antibody.)

Rabbit UPF1 Polyclonal Antibody | anti-UPF1 antibody

UPF1 (UP Frameshift 1, FLJ43809, FLJ46894, HUPF1, KIAA0221, NORF1, Regulator of Nonsense Transcripts 1, RENT1, pNORF1) (HRP)

Gene Names
UPF1; HUPF1; NORF1; RENT1; smg-2; pNORF1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified by affinity chromatography.
Synonyms
UPF1; Polyclonal Antibody; UPF1 (UP Frameshift 1; FLJ43809; FLJ46894; HUPF1; KIAA0221; NORF1; Regulator of Nonsense Transcripts 1; RENT1; pNORF1) (HRP); anti-UPF1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes UPF1. Species Crossreactivity: human, mouse, rat
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-UPF1 antibody
Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
IF: 1:50-1:200
WB: 1:500-1:2000
IHC: 1:50-1:200
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to human UPF1.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using UPF1 antibody.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using UPF1 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using UPF1 antibody. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cell using UPF1 antibody. Blue: DAPI for nuclear staining.)
Product Categories/Family for anti-UPF1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
1129
NCBI Official Full Name
Regulator of nonsense transcripts 1
NCBI Official Synonym Full Names
UPF1 regulator of nonsense transcripts homolog (yeast)
NCBI Official Symbol
UPF1
NCBI Official Synonym Symbols
HUPF1; NORF1; RENT1; smg-2; pNORF1
NCBI Protein Information
regulator of nonsense transcripts 1; delta helicase; yeast Upf1p homolog; ATP-dependent helicase RENT1; nonsense mRNA reducing factor 1; up-frameshift mutation 1 homolog; up-frameshift suppressor 1 homolog; smg-2 homolog, nonsense mediated mRNA decay fact
UniProt Protein Name
Regulator of nonsense transcripts 1
Protein Family
UniProt Gene Name
UPF1
UniProt Synonym Gene Names
KIAA0221; RENT1; NORF1; hUpf1
UniProt Entry Name
RENT1_HUMAN

NCBI Description

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

RENT1: RNA-dependent helicase and ATPase required for nonsense- mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1- eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD. Essential for embryonic viability. Found in a post-splicing messenger ribonucleoprotein (mRNP) complex. Associates with the exon junction complex (EJC). Associates with the SGM1C complex; is phosphorylated by the complex kinase component SGM1. Interacts with UPF2, UPF3A and UPF3B. Interacts with EST1A and SLBP. Interacts (when hyperphosphorylated) with PNRC2. Interacts with EIF2C1, EIF2C2 and GSPT2. Ubiquitous. Belongs to the DNA2/NAM7 helicase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; C2H2-type zinc finger protein; EC 3.6.4.-; Hydrolase; EC 3.6.1.-

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus; chromatin

Molecular Function: protein binding; DNA binding; zinc ion binding; RNA binding; chromatin binding; helicase activity; ATP binding; ATP-dependent RNA helicase activity

Biological Process: mRNA export from nucleus; dosage compensation, by inactivation of X chromosome; mRNA catabolic process, nonsense-mediated decay; gene expression; DNA replication; DNA repair; regulation of translational termination

Research Articles on UPF1

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Product Notes

The UPF1 upf1 (Catalog #AAA6442615) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UPF1 (UP Frameshift 1, FLJ43809, FLJ46894, HUPF1, KIAA0221, NORF1, Regulator of Nonsense Transcripts 1, RENT1, pNORF1) (HRP) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's UPF1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC). IF: 1:50-1:200 WB: 1:500-1:2000 IHC: 1:50-1:200 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the UPF1 upf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UPF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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