Mouse Coagulation factor X ELISA Kit | FX elisa kit

Mouse Coagulation factor X ELISA Kit

Cat. Num. AAA764671

• Gene Names: F10; FX; FXA
• Reactivity: Mouse
• Synonyms: Coagulation factor X; Mouse Coagulation factor X ELISA Kit; FX/F10/Coagulation Factor X/Cf10/FXa/Prothrombinase/Stuart Factor/factor Xa/FXA/Stuart-Prower factor; FX elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of FX. No significant cross-reactivity or interference between FX and analogues was observed.
• Sequence Length: 488

• Samples: Plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.938-60ng/ml
• Sensitivity: 0.563ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FX elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4503625
• NCBI GeneID: 2159
• NCBI Accession #: NP_000495.1
• NCBI GenBank Nucleotide #: NM_000504.3
• Molecular Weight: 54,732 Da
• NCBI Official Full Name: coagulation factor X isoform 1 preproprotein
• NCBI Official Synonym Full Names: coagulation factor X
• NCBI Official Symbol: F10
• NCBI Official Synonym Symbols: FX; FXA
• NCBI Protein Information: coagulation factor X
• UniProt Protein Name: Coagulation factor X
• Protein Family: Coagulation factor
• UniProt Gene Name: F10
• UniProt Entry Name: FA10_HUMAN

NCBI Description

This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides. [provided by RefSeq, Aug 2015]

Uniprot Description

F10: Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. Defects in F10 are the cause of factor X deficiency (FA10D). A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis. Belongs to the peptidase S1 family.

Protein type: Secreted; EC 3.4.21.6; Motility/polarity/chemotaxis; Lipid-binding; Secreted, signal peptide; Protease

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: endoplasmic reticulum lumen; extracellular region; Golgi lumen; plasma membrane

Molecular Function: calcium ion binding; phospholipid binding; protein binding; serine-type endopeptidase activity

Biological Process: blood coagulation; blood coagulation, extrinsic pathway; blood coagulation, intrinsic pathway; cellular protein metabolic process; ER to Golgi vesicle-mediated transport; peptidyl-glutamic acid carboxylation; positive regulation of cell migration; positive regulation of protein kinase B signaling cascade; post-translational protein modification; signal peptide processing

Disease: Factor X Deficiency